In vitro and in vivo evaluation of the bile acid-binding properties of dried persimmon and its non-extractable proanthocyanidin fraction
In vitro and in vivo evaluation of the bile acid-binding properties of dried persimmon and its non-extractable proanthocyanidin fraction
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干柿及其不可提取原花青素组分胆汁酸结合特性的体外和体内评价
DOI：
10.1016/j.foodchem.2021.131617
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发表时间：
2022
期刊：
影响因子：
8.8
通讯作者：
Ikeda Erika
中科院分区：
文献类型：
--
作者：
Hamauzu Yasunori;Ikeda Erika
干柿子含有丰富的不溶性单宁组成的不可提取的原花青素（NEPAs）。为了确定NEPAs的有益功能，我们使用体外和体内实验研究了NEPAs的胆汁酸结合能力。为了进行体外评价，从干燥过程中的柿子中制备了具有不同NEPA水平的70%（v/v）丙酮不溶性固体（AIS）。具有较高NEPA水平的AIS显示出更强的胆汁酸结合活性，并且最高NEPA水平可以从大多数干燥的水果中获得。通过对C57 BL/6 J小鼠的饲养试验，验证了柿饼粉和AIS的排胃酸作用。平均胆汁酸排泄量顺序为低脂组（1.07 µmol/d）<高脂（HF）组（3.03 µmol/d）< HF + AIS组（4.44 µmol/d）< HF + DP组（6.74 µmol/d）。这些结果表明，果实的胆汁酸排泄功能与NEPAs的存在有关，NEPAs是柿饼的组成成分。
Dried persimmons contain abundant insoluble tannins comprised of non-extractable proanthocyanidins (NEPAs). To determine the beneficial function of NEPAs, we studied the bile acid-binding ability of NEPAs usingin vitroandin vivoexperiments. For thein vitroevaluation, 70% (v/v) acetone-insoluble solids (AIS) with different NEPA levels was prepared from persimmon fruits during drying. AIS with higher NEPA levels displayed stronger bile acid-binding activity, and the highest NEPA levels can be obtained from mostly-dried fruits. Thein vivobile acid-excreting effect of dried persimmon powder (DP) and AIS was verified by feeding experiments using C57BL/6J mice. The average bile acid-excretion was in order of low-fat group (1.07 µmol/day) < high-fat (HF) group (3.03 µmol/day) < HF + AIS group (4.44 µmol/day) < HF + DP group (6.74 µmol/day). These results suggest that the bile acid-excreting function of the fruit is related to the presence of NEPAs, which are constitutive ingredients of dried persimmon.