CELL-SUSPENSION AND PROTOPLAST CULTURE IN RICE
CELL-SUSPENSION AND PROTOPLAST CULTURE IN RICE
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DOI：
10.1016/0168-9452(85)90086-x
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发表时间：
1985-11-01
期刊：
影响因子：
5.2
通讯作者：
HINATA, K
中科院分区：
文献类型：
--
作者：
TORIYAMA, K;HINATA, K
以氨基酸混合物为唯一氮源，在AA培养基中获得了分散良好的水稻花药愈伤组织细胞悬液。AA培养基能有效促进原生质体的释放。这些原生质体在B5培养基中培养8 ~ 10 d后转移到AA培养基中，细胞持续分裂，愈伤组织产生频率高。原生质体培养的关键因素似乎是培养基中的氮源。原生质体愈伤组织可形成绿斑和根。
A finely dispersed cell suspension was obtained from anther-derived rice callus in AA medium, which contained an amino acid mixture as the sole nitrogen source. AA medium promoted the release of protoplasts effectively. These protoplasts, when cultured initially in B5 medium for 8-10 days followed by transfer to AA medium, showed sustained cell divisions and gave rise to callus with a high frequency. The critical factor in the protoplast culture seemed to be the nitrogen source in the culture medium. Green-spot and root formation could be obtained from the protoplast-derived callus.