Clues from planarians about interleukin-17A and stress that result from light avoidance: IL-17A antagonists reduce defensive responding in flatworms.
Clues from planarians about interleukin-17A and stress that result from light avoidance: IL-17A antagonists reduce defensive responding in flatworms.
复制标题
涡虫关于白细胞介素 17A 和避光引起的应激的线索：IL-17A 拮抗剂可降低扁虫的防御反应。
DOI：
10.1016/j.cyto.2023.156345
复制
发表时间：
2023
期刊：
影响因子：
3.8
通讯作者：
Rawls,ScottM
中科院分区：
文献类型：
--
作者：
Milton,Mia;Inan,Saadet;Rawls,ScottM
新出现的证据将白细胞介素-17A（IL-17 A）与焦虑和压力联系起来。在患有焦虑症的患者中，IL-17 A的循环水平升高，并且IL-17信号传导的药理学阻断或IL-17的遗传缺失减少小鼠中的焦虑样行为。鉴于IL-17是动物门中最保守的细胞因子之一，我们测试了抗IL-17治疗减少涡虫中的防御反应的假设，涡虫是具有双侧对称性和具有头部化的CNS的最简单的动物。选择的终点是光回避，这是一种常见的表型的涡虫和啮齿动物和防御反应的指数，这是减少抗焦虑化合物在这两个物种。将Planarians放置在含有水或测试溶液的皮氏培养皿的中线处，所述皮氏培养皿均等地分成亮和暗两半。Planarians暴露于选择性IL-17 A抗体（0.1，1，10 pM）超过5分钟的时间间隔花更多的时间在光比水暴露的Planarians。矢车菊素（0.01，0.11，10 µM），一种抗炎类黄酮和非选择性IL-17 A抑制剂，也增加了在光中花费的时间。IL-17 A抗体或矢车菊素在减少光回避的浓度下不影响运动性，尽管更高的浓度会降低运动性（>10 µM）。我们的研究结果表明，IL-17 A拮抗剂减少了防御反应，并建议保护IL-17 A方面的焦虑相关行为的影响。
Emerging evidence links interleukin-17A (IL-17A) to anxiety and stress. Circulating levels of IL-17A are elevated in patients with anxiety disorders, and pharmacological blockade of IL-17 signaling or genetic deletion of IL-17 reduces anxiety-like behaviors in mice. Given that IL-17 is one of the most conserved cytokines among animal phyla, we tested the hypothesis that anti-IL-17 treatments reduce defensive responding in planarians, the simplest animal with bilateral symmetry and a CNS with cephalization. The endpoint selected was light avoidance, which is a common phenotype of planarians and rodents and an index of defensive responding that is reduced by anxiolytic compounds in both species. Planarians were placed at the midline of a Petri dish containing water or test solution that was equally split into light and dark halves. Planarians exposed to a selective IL-17A antibody (0.1, 1, 10 pM) over a 5-min interval spent more time in the light than water-exposed planarians. Cyanidin (0.01, 0.1 1, 10 µM), an anti-inflammatory flavonoid and non-selective IL-17A inhibitor, also increased time spent in the light. Motility was not affected by IL-17A antibody or cyanidin at concentrations that reduced light avoidance, although higher concentrations reduced motility (>10 µM). Our results show that IL-17A antagonists reduce defensive responding in planarians and suggest conservation of IL-17A effects on aspects of anxiety-related behaviors.