MALDI-TOF Mass Array Analysis of Nell-1 Promoter Methylation Patterns in Human Gastric Cancer.
MALDI-TOF Mass Array Analysis of Nell-1 Promoter Methylation Patterns in Human Gastric Cancer.
复制标题
人胃癌中 Nell-1 启动子甲基化模式的 MALDI-TOF 质谱分析。
DOI：
10.1155/2015/136941
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发表时间：
2015
影响因子：
--
通讯作者：
Zhang Q
中科院分区：
文献类型：
--
作者：
Gao C;Zhang Q;Kong D;Wu D;Su C;Tong J;Chen F;Zhang Q
质谱学(MS)可以对生物分子(蛋白质、多肽、寡糖、脂类、DNA和RNA)、药物和代谢物进行快速、灵敏的定性和定量分析。质谱仪已成为现代生物医学研究的重要工具，包括DNA甲基化分析。DNA甲基化在许多癌症中都有报道，这表明它可以作为早期生物标志物来提高早期诊断率。利用基质辅助激光解吸电离飞行时间质谱仪和MassCLEAVE试剂，我们比较了胃癌患者肿瘤组织、癌旁组织和正常组织中Nell-1的甲基化水平。扩增产物中近80%的CpG位点被分析所覆盖。我们的结果表明，胃癌组织(较高水平)和正常组织的甲基化状态有显著差异。在胃癌组织和癌旁组织中也发现了同样的趋势。实时荧光定量聚合酶链式反应检测到Nell-1的相对低表达。此外，免疫组织化学分析显示，与正常组织相比，Nell-1在癌症组织中的染色强度较低，肿瘤细胞已扩散到肌肉层。这些发现对胃癌的早期诊断有一定的指导意义。
Mass spectrometry (MS) enables rapid and sensitive qualitative and quantitative analyses of biomolecules (proteins, peptides, oligosaccharides, lipids, DNA, and RNA), drugs, and metabolites. MS has become an essential tool in modern biomedical research, including the analysis of DNA methylation. DNA methylation has been reported in many cancers, suggesting that it can be utilized as an early biomarker to improve the early diagnosis rate. Using matrix-assisted laser desorption/ionization time-of-flight MS and MassCLEAVE reagent, we compared Nell-1 hypermethylation levels among tumor tissues, paracarcinoma tissues, and normal tissues from gastric cancer patients. Almost 80% of the CpG sites in the amplicons produced were covered by the analysis. Our results indicate a significant difference in methylation status between gastric cancer tissue (a higher level) and normal tissue. The same trend was identified in gastric cancer tissue versus paracarcinoma tissue. We also detected lower relative expression of Nell-1 by real-time PCR. Furthermore, immunohistochemical analyses revealed that Nell-1 staining was less intense in cancer tissue relative to normal tissue and that the tumor cells had spread to the muscle layer. These findings may serve as a guide for the early diagnosis of gastric cancer.
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影响因子：
3.7
作者：
Peng DF;Hu TL;Schneider BG;Chen Z;Xu ZK;El-Rifai W
通讯作者：
El-Rifai W
DOI：
10.1016/s0140-6736(09)60617-6
发表时间：
2009-08-08
期刊：
Lancet (London, England)
影响因子：
--
作者：
Hartgrink HH;Jansen EP;van Grieken NC;van de Velde CJ
通讯作者：
van de Velde CJ
影响因子：
64.5
作者：
Li, QL;Ito, K;Ito, Y
通讯作者：
Ito, Y
影响因子：
29.4
作者：
Wanajo, Aira;Sasaki, Akane;Akiyama, Yoshimitsu
通讯作者：
Akiyama, Yoshimitsu
DOI：
10.1073/pnas.0712251105
发表时间：
2008-03-25
影响因子：
11.1
作者：
Ehrich, Mathias;Turner, Julia;van den Boom, Dirk
通讯作者：
van den Boom, Dirk