A quantitative proteomic analysis of mitochondrial participation in p19 cell neuronal differentiation.
A quantitative proteomic analysis of mitochondrial participation in p19 cell neuronal differentiation.
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DOI：
10.1021/pr070300g
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发表时间：
2008
影响因子：
4.4
通讯作者：
J. Watkins;S. Basu;D. Bogenhagen
中科院分区：
文献类型：
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作者：
J. Watkins;S. Basu;D. Bogenhagen
定量蛋白质组学分析的蛋白质表达的变化伴随着分化的P19小鼠胚胎癌细胞成神经元样细胞，使用同量异序标签技术结合LC-MS/MS揭示蛋白质的变化，反映退出细胞周期伴随着动态重组的细胞骨架和上调线粒体生物合成。对纯化的线粒体组分中单个蛋白质丰度的定量变化的进一步研究表明，大多数线粒体蛋白质的丰度显著增加。一组伴侣蛋白没有参与这种增加，这表明神经元样细胞是相对缺乏线粒体伴侣。我们开发了一种程序，以解释在不同的细胞或组织来源的细胞器的纯化过程中线粒体蛋白质的回收差异。RT-PCR分析支持的蛋白质组学数据表明，在神经元分化过程中增强的线粒体生物合成可能反映了PGC-1 α表达的大幅增加及其负调节因子p160 Mybbp 1a的下调。
A quantitative proteomic analysis of changes in protein expression accompanying the differentiation of P19 mouse embryonal carcinoma cells into neuron-like cells using isobaric tag technology coupled with LC-MS/MS revealed protein changes reflecting withdrawal from the cell cycle accompanied by a dynamic reorganization of the cytoskeleton and an up-regulation of mitochondrial biogenesis. Further study of quantitative changes in abundance of individual proteins in a purified mitochondrial fraction showed that most mitochondrial proteins increased significantly in abundance. A set of chaperone proteins did not participate in this increase, suggesting that neuron-like cells are relatively deficient in mitochondrial chaperones. We developed a procedure to account for differences in recovery of mitochondrial proteins during purification of organelles from distinct cell or tissue sources. Proteomic data supported by RT-PCR analysis suggests that enhanced mitochondrial biogenesis during neuronal differentiation may reflect a large increase in expression of PGC-1alpha combined with down-regulation of its negative regulator, p160 Mybbp1a.