New cytotoxic spectinabilin derivative from ant-associated Streptomyces sp. 1H-GS5
New cytotoxic spectinabilin derivative from ant-associated Streptomyces sp. 1H-GS5
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DOI：
10.1038/ja.2015.99
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发表时间：
2015-09
期刊：
影响因子：
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通讯作者：
Shuang Liu;Mei-dong Xu;Hui Zhang;Huan Qi;Ji Zhang;Chongxi Liu;Jidong Wang;W. Xiang;Xiangjing Wang
中科院分区：
文献类型：
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作者：
Shuang Liu;Mei-dong Xu;Hui Zhang;Huan Qi;Ji Zhang;Chongxi Liu;Jidong Wang;W. Xiang;Xiangjing Wang
昆虫是地球上最丰富多样的动物类别，它们与各种各样的微生物有关。昆虫在独特的生态位栖息地中生活的能力通常由其相关的微生物促进，1这些微生物通过产生代谢物来执行许多重要的宿主功能，特别是对抗病原体，寄生虫或捕食者2，3并且巨大的昆虫相关微生物群落是新型抗生素代谢物的潜在来源。4、5为了寻找更多的昆虫伴生微生物衍生的生物活性代谢产物，对木蚁伴生微生物进行了研究，获得了一株对某些肿瘤细胞株具有良好活性的链霉菌1H-GS 5。在进一步研究链霉菌属1H-GS 5发酵液的化学成分时，分离出一种新的spectinabilin衍生物（1）沿着已知化合物spectinabilin（2）（图1）。从东北农业大学采集的日本弓背蚁（Camponotus japonicas Mayr）头上分离得到一株链霉菌（Streptomyces sp.）1H-GS 5，该菌株具有良好的生物活性。该菌株的16 S rRNA序列（GenBank登录号：KP 784764）与Streptomycesabiabilis NBRC 13424（登录号：AB 184393）的16 S rRNA序列相似性高达99%，因此鉴定为链霉菌属。链霉菌属1H-GS 5菌株保藏于浙江海正集团有限公司药物研究培养物保藏中心，Ltd，保藏号：HS-HX-087。将菌株保持在YMS培养基上，该培养基含有可溶性淀粉（Bei Jing Ao Bo Xing，Beijing，China）10 g、酵母提取物（Bei Jing Ao Bo Xing）2 g、KNO 3 1 g和琼脂20 g，在1.0 l自来水中，pH 7.0。种子培养基由葡萄糖（BeijingAoboXing）20 g、大豆粉（Cormwin，Beijing，China）15 g和酵母自溶物（BeijingAoboXing）5.0 g在1.0 l自来水中组成，pH 7.0。两个媒体都是
Insects are the most abundant and diverse animal class on earth and they are associated with an amazing variety of microorganisms. The ability of insects to live in unique niche habitats is often facilitated by their associated microbe, 1 which perform many important host functions, especially against pathogens, parasitoids or predators by producing metabolites 2, 3 and the enormous insect-associated microbial communities are a potential source of novel antibiotic metabolites. 4, 5 To find more insect-associated microorganisms-derived bioactive metabolites, the study of the carpenter ants-associated microorganisms was carried out and a strain Streptomyces sp. 1H-GS5 exhibiting good activities against some lines of tumor cell was obtained. In the further investigation of chemical components from the broth of Streptomyces sp. 1H-GS5, a new spectinabilin derivative (1) along with the known compound, spectinabilin (2)(Figure 1), were isolated. Here the report deals with the details of the isolation, structure elucidation and the bioactivity of the new compound.The strain Streptomyces sp. 1H-GS5 was isolated from the head of ant (Camponotus japonicas Mayr) collected from the Northeast Agricultural University, Harbin of Heilongjiang province, China. The strain was identified as the genus Streptomyces because its 16S rRNA sequence (accession no: KP784764 in the GenBank, National Center for Biotechnology Information) exhibited a high-sequence similarity of 99% with that of Streptomyces spectabilis NBRC 13424 (accession no: AB184393). The strain Streptomyces sp. 1H-GS5 has been deposited in the Pharmaceutical Research Culture Collection, Zhejiang Hisun Group Co., Ltd, with accession no: HS-HX-087. Strain was maintained on a YMS medium containing soluble starch (Bei Jing Ao Bo Xing, Beijing, China) 10 g, yeast extract (Bei Jing Ao Bo Xing) 2 g, KNO3 1 g and agar 20 g in 1.0 l tap water, pH 7.0. The seed medium consisted of glucose (Bei Jing Ao Bo Xing) 20 g, soybean flour (Cormwin, Beijing, China) 15 g and yeast autolysate (Bei Jing Ao Bo Xing) 5.0 g in 1.0 l tap water, pH 7.0. Both the media were