Enhancement of growth by expression of poplar cellulase in Arabidopsis thaliana
Enhancement of growth by expression of poplar cellulase in Arabidopsis thaliana
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DOI：
10.1046/j.1365-313x.2003.01696.x
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发表时间：
2003-03-01
期刊：
影响因子：
7.2
通讯作者：
Hayashi, T
中科院分区：
文献类型：
--
作者：
Park, YW;Tominaga, R;Hayashi, T
为了研究纤维素酶和纤维素酶在植物生长中的作用，我们在拟南芥中结构性表达了杨树纤维素酶(PaPopCel1)。由于细胞大小的增加，表达增加了玫瑰花环的大小。生长的变化伴随着生物力学性质的变化，通过化学分析和核磁共振(核磁共振)谱表明，细胞壁结构表明与纤维素微纤维交联的木葡聚糖减少。这一结果支持了杨树纤维素酶攻击纤维素微纤维的晶旁位置以疏松木聚糖插层的观点，这种不可逆壁修饰促进了植物细胞的放大。
To study the role of cellulose and cellulase in plant growth, we expressed poplar cellulase (PaPopCel1 ) constitutively in Arabidopsis thaliana . Expression increased the size of the rosettes due to increased cell size. The change in growth was accompanied by changes in biomechanical properties due to cell wall structure indicative of decrease in xyloglucan cross-linked with cellulose microfibrils by chemical analysis and nuclear magnetic resonance (NMR) spectra. The result supports the concept that the paracrystalline sites of cellulose microfibrils are attacked by poplar cellulase to loosen xyloglucan intercalation and this irreversible wall modification promotes the enlargement of plant cells.