Designed dendrimer syntheses by self-assembly of single-site, ssDNA functionalized dendrons
Designed dendrimer syntheses by self-assembly of single-site, ssDNA functionalized dendrons
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DOI：
10.1021/nl034957m
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发表时间：
2004-05-01
期刊：
影响因子：
10.8
通讯作者：
Tomalia, DA
中科院分区：
文献类型：
--
作者：
DeMattei, CR;Huang, BH;Tomalia, DA
通过将互补的32个碱基对寡核苷酸共价缀合至具有中性或阴离子表面基团的单位点、硫醇官能化的树状-PAMAM二-树枝状分子，已经合成了单位点、官能化的单链（ssDNA）树状-聚（酰胺基胺）（PAMAM）二-树枝状分子。在适当的组装温度下组合这些互补（ss-DNA）官能化的PAMAM二树枝化基元产生沃森-克里克碱基配对（dsDNA）核心，其被四个PAMAM树枝化基元包围。这些新的核-壳纳米结构代表了一类新的精确的单分散，线性树枝状建筑共聚物。使用比较凝胶电泳，它被证明，这些自组装（二树枝状）树枝状聚合物可以半球分化作为一个功能的表面化学以及世代大小。这种新的超大分子的方法提供了一个非常容易和通用的策略的大小，形状和表面取代基的组合设计均匀和分化的树枝状纳米结构。
Single site, functionalized, single stranded (ssDNA) dendri-poly(amidoamine) (PAMAM) di-dendrons have been synthesized by covalently conjugating complementary 32 base pair oligonucleotides to single-site, thiol functionalized dendri-PAMAM di-dendrons possessing neutral or anionic surface groups. Combining these complementary (ss-DNA) functionalized PAMAM di-dendrons at appropriate assembly temperatures produced Watson-Crick base paired (dsDNA) cores, surrounded by four PAMAM dendrons. These novel core-shell nanostructures represent a new class of precise monodisperse, linear-dendritic architectural copolymers. Using comparative gel electrophoresis, it was demonstrated that these self-assembled (di-dendron) dendrimers could be hemispherically differentiated as a function of surface chemistry as well as generational size. This new supramacromolecular approach offers a very facile and versatile strategy for the combinatorial design of size, shape, and surface substituents for both homogeneous and differentiated dendritic nanostructures.