Colocalization of oestrogen receptor immunoreactivity and preproenkephalin mRNA expression to neurons in the superficial laminae of the spinal and medullary dorsal horn of rats
Colocalization of oestrogen receptor immunoreactivity and preproenkephalin mRNA expression to neurons in the superficial laminae of the spinal and medullary dorsal horn of rats
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DOI：
10.1111/j.1460-9568.1996.tb01207.x
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发表时间：
1996-11-01
影响因子：
3.4
通讯作者：
Blomqvist, A
中科院分区：
文献类型：
--
作者：
Amandusson, A;Hermanson, O;Blomqvist, A
采用放射性标记的cRNA探针结合免疫组织化学染色和原位杂交的双标记方法，证明雌激素受体样免疫反应性和前脑啡肽原-A mRNA在雌性大鼠的延髓和脊髓背角。这两种标记物均标记胶状质及其三叉神经同源物中的大量神经元，其中许多神经元为双标记，既显示雌激素受体样免疫反应性，又显示前脑啡肽原A mRNA;细胞计数显示，浅层中40-60%的雌激素受体样免疫反应细胞也被前脑啡肽原-AmRNA标记，在同一板层中60-70%的前脑啡肽原-A mRNA标记神经元显示雌激素受体样免疫反应性。以前的研究表明，雌激素受体可以结合到前脑啡肽原-A基因的启动子区，下丘脑的研究表明，雌激素调节脑啡肽在选定的神经元群体的表达。本研究结果表明，脑啡肽能神经元的浅背角含有雌激素受体，并建议雌激素可能发挥重要作用的调制感觉和伤害性的处理在下延髓和脊髓。
A double-labelling procedure combining immunohistochemical staining with in situ hybridization using a radiolabelled cRNA probe was employed to demonstrate oestrogen receptor-like immunoreactivity and preproenkephalin-A mRNA in the medullary and spinal dorsal horn of female rats. Both markers labelled large numbers of neurons in the substantia gelatinosa and its trigeminal homologue, Many of these neurons were double-labelled, displaying both oestrogen receptor-like-immunoreactivity and preproenkephalin-A mRNA; cell counts showed that 40-60% of the of the oestrogen receptor-like-immunoreactive cells in the superficial laminae also were labelled for preproenkephalin-A mRNA, and that 60-70% of the preproenkephalin-A mRNA-labelled neurons in the same laminae displayed oestrogen receptor-like immunoreactivity. Previous studies have shown that oestrogen receptors can bind to the promoter region of the preproenkephalin-A gene, and studies on the hypothalamus have demonstrated that oestrogen regulates enkephalin expression in select neuronal populations. The present results demonstrate that enkephalinergic neurons in the superficial dorsal horn contain oestrogen receptors and suggest that oestrogen may play an important role in the modulation of sensory and nociceptive processing in the lower medulla and spinal cord.