A new human gene KCNRG encoding potassium channel regulating protein is a cancer suppressor gene candidate located in 13q14.3
A new human gene KCNRG encoding potassium channel regulating protein is a cancer suppressor gene candidate located in 13q14.3
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DOI：
10.1016/s0014-5793(03)00211-4
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发表时间：
2003-03-27
期刊：
影响因子：
3.5
通讯作者：
Baranova, AV
中科院分区：
文献类型：
--
作者：
Ivanov, DV;Tyazhelova, TV;Baranova, AV
我们报告了定位于染色体13q14.3带的一个新基因KCNRG的初步特征。该基因包括三个外显子，并具有两个在正常组织和一些肿瘤细胞系中表达的选择性剪接异构体。KCNRG蛋白与电压门控性钾通道的四聚体结构域具有高度同源性。采用膜片钳技术，我们确定KCNRG抑制人前列腺细胞系LNCaP的K+通道活性。已知K+通道的选择性阻断剂抑制淋巴细胞和LNCaP细胞系增殖。我们认为KCNRG是B细胞慢性淋巴细胞白血病和前列腺癌肿瘤抑制基因的候选者。(C)2003年由Elsevier Science B.V.代表欧洲生物化学学会联合会出版。
We report the primary characterization of a new gene KCNRG mapped at chromosome band 13q14.3. This gene includes three exons and has two alternatively spliced isoforms that are expressed in normal tissues and in some tumor cell lines. Protein KCNRG has high homology to tetramerization domain of voltage-gated K+ channels. Using the patch-clamp technique we determined that KCNRG suppresses K+ channel activity in human prostate cell line LNCaP. It is known that selective blockers of K+ channels suppress lymphocyte and LNCaP cell line proliferation. We suggest that KCNRG is a candidate for a B-cell chronic lymphocytic leukemia and prostate cancer tumor suppressor gene. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.