Mobility of synaptic vesicles in different pools in resting and stimulated frog motor nerve terminals
Mobility of synaptic vesicles in different pools in resting and stimulated frog motor nerve terminals
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DOI：
10.1016/j.neuron.2006.06.031
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发表时间：
2006-08-03
期刊：
影响因子：
16.2
通讯作者：
Betz, William J.
中科院分区：
文献类型：
--
作者：
Gaffield, Michael A.;Rizzoli, Silvio O.;Betz, William J.
我们使用荧光恢复后光漂白（FRAP）来衡量突触囊泡在蛙运动神经末梢的流动性。属于回收池或储备池的囊泡用FM 1 -43选择性标记。在静息终末，储备池中的囊泡是不移动的，而再循环池中的囊泡是移动的。神经刺激增加储备池囊泡的流动性。治疗latrunculin A，破坏肌动蛋白丝，没有显着的影响，流动性，降低温度同样也没有什么影响，这表明回收池囊泡移动简单的扩散。冈田酸的应用引起囊泡的流动性在两个池中增加到相同的水平。我们可以通过考虑每个池中移动的和非移动的囊泡的相对数量以及对移动性有很大影响的囊泡堆积密度来定量地模拟这些和其他结果。
We used fluorescence recovery after photobleaching (FRAP) to measure the mobility of synaptic vesicles in frog motor nerve terminals. Vesicles belonging to the recycling pool or to the reserve pool were selectively labeled with FM1-43. In resting terminals, vesicles in the reserve pool were immobile, while vesicles in the recycling pool were mobile. Nerve stimulation increased the mobility of reserve pool vesicles. Treatment with latrunculin A, which destroyed actin filaments, had no significant effect on mobility, and reducing the temperature likewise had little effect, suggesting that recycling pool vesicles move by simple diffusion. Application of okadaic acid caused vesicle mobility in both pools to increase to the same level. We could model these and others' results quantitatively by taking into account the relative numbers of mobile and immobile vesicles in each pool, and vesicle packing density, which has a large effect on mobility.