Phosphate is required for inhibition by glucose of development of hamster 8-cell embryos in vitro.
Phosphate is required for inhibition by glucose of development of hamster 8-cell embryos in vitro.
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葡萄糖抑制仓鼠 8 细胞胚胎体外发育需要磷酸盐。
DOI：
10.1095/biolreprod40.3.607
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发表时间：
1989
影响因子：
3.6
通讯作者：
Bavister,BD
中科院分区：
文献类型：
--
作者：
Seshagiri,PB;Bavister,BD
采用改良Tyrode培养基TLP-PVA，研究了磷酸二氢钠（Pi）和葡萄糖对丙酮酸(P)、氨基酸(A)或乳酸(L)介导的仓鼠8细胞胚胎发育的影响。当丙酮酸作为TP-PVA培养基中唯一的能量底物用于胚胎发育时，无论培养基中是否含有0.35 mM Pi或5 mM葡萄糖，囊胚形成率都在81.3 ~ 90.9%之间；但是，当这两种化合物同时存在时，囊胚形成率下降到51.8%。同样，在含有四种氨基酸（Phe、Ile、Met和Gln）的TA-PVA培养基中，无论培养基中是否含有0.35 mM Pi或5 mM葡萄糖，胚胎发育成囊胚的比例都在74.1%至90.4%之间；但是，当这些化合物同时存在时，囊胚形成率下降到16.0%。在TL-PVA培养基中，10 mM乳酸钠支持胚胎发育（84.4%囊胚）；0.35 mM Pi的添加使囊胚发育率降低至65.6%。然而，在无pi的TL-PVA培养基中添加葡萄糖并没有减少囊胚的形成（81.3%）；当培养基中含有0.35 mM Pi时，葡萄糖使囊胚发育减少到7.5%。当葡萄糖和Pi在不同浓度下相互作用时，葡萄糖在不含Pi的hl - pva培养基中不受抑制（74.3%囊胚），但0.25 mM葡萄糖在0.35 mM Pi存在下显著抑制胚胎发育（7.7%囊胚）。即使在没有葡萄糖的情况下，相对高浓度（1 mM）的磷酸盐也具有抑制作用（37.9%）。这些结果表明，Pi在培养基中的存在是抑制葡萄糖作用的必要条件。在含有Pi和葡萄糖的培养基中，只有当培养基中含有丙酮酸而不含乳酸或氨基酸时，仓鼠8细胞胚胎才能发育（囊胚≥50%）。当测试一种不可糖酵解的葡萄糖类似物，即2-脱氧葡萄糖，对乳酸介导的胚胎发育有抑制作用时，无论培养基中是否含有Pi。结果表明，Pi在体外调节葡萄糖对仓鼠8细胞胚胎发育的抑制作用。
The influence of sodium dihydrogen phosphate (Pi) and glucose on the development of hamster 8-cell embryos mediated by pyruvate (P) or amino acids (A) or lactate (L) was investigated using modified Tyrode’s medium, TLP-PVA. When pyruvate was tested as the only energy substrate in medium TP-PVA for embryo development, blastocyst formation ranged from 81.3 to 90.9% whether or not the medium contained 0.35 mM Pi or 5 mM glucose; but, when these two compounds were present together, blastocyst formation fell to 51.8%. Similarly, in TA-PVA medium containing four amino acids: Phe, Ile, Met, and Gln), embryo development to blastocyst ranged from 74.1% to 90.4% whether or not the medium contained 0.35 mM Pi or 5 mM glucose; but, when these compounds were present together, blastocyst formation fell to 16.0%. In TL-PVA medium, 10 mM sodium lactate supported embryo development (84.4% blastocysts); the addition of 0.35 mM Pi decreased blastocyst development to 65.6%. However, addition of glucose to Pi-free TL-PVA medium did not decrease blastocyst formation (81.3%); when the medium contained 0.35 mM Pi, glucose curtailed blastocyst development to 7.5%. When glucose and Pi interactions were studied at different concentrations, glucose up to 1 mM was not inhibitory in Pi-free TL-PVA medium (74.3% blastocysts), but 0.25 mM glucose in the presence of 0.35 mM Pi markedly inhibited embryo development (7.7% blastocysts). Phosphate at a relatively high concentration (1 mM) was inhibitory (37.9% blastocysts), even in the absence of glucose. These results indicate that the presence of Pi in the culture medium is obligatory for the inhibitory effect of glucose. In medium containing both Pi and glucose, hamster 8-cell embryo development (≥ 50% blastocysts) can occur only if the medium contains pyruvate but not lactate or amino acids. When a non-glycolysable glucose analogue, i.e. 2-deoxy-Dglucose, was tested on the lactate-mediated embryo development, it was inhibitory whether or not the medium contained Pi. The data reveal that Pi regulates the glucose inhibition of development of hamster 8-cell embryos in vitro.