Establishment and characterization of GSA-1, a human cell line highly susceptible to apoptosis after free-fall.
Establishment and characterization of GSA-1, a human cell line highly susceptible to apoptosis after free-fall.
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GSA-1（一种在自由落体后极易发生细胞凋亡的人类细胞系）的建立和表征。
DOI：
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发表时间：
2002
影响因子：
2
通讯作者：
N. Suzuki
中科院分区：
文献类型：
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作者：
J. Nomura;Jyuni Himeda;Zheng Chen;S. Sugaya;Shunji Takahashi;K. Kita;M. Ichinose;N. Suzuki
微重力和/或重力变化应力诱导的细胞凋亡被认为是细胞死亡的重要原因之一，尽管细胞凋亡事件的分子机制仍不清楚。在这项研究中，我们建立了一个细胞系，GSA-1，从甲基磺酸乙酯处理的人RSa细胞。GSA-1细胞在自由落体后对凋亡高度敏感;这些细胞中有24.4%在自由落体后发生凋亡，而RSa细胞仅为6%。GSA-1细胞的凋亡增强紫外线（UV，主要是254 nm波长）照射前自由落体到更大的程度比RSa细胞。细胞凋亡的分子机制包括p53和Bax蛋白;在GSA-1细胞中，无论照射与否，在自由落体后4 h，细胞核p53和细胞质Bax的表达增加。此外，去除率的环丁烷嘧啶二聚体（CPD）在紫外线照射的GSA-1细胞中的细胞暴露于自由落体比在1-G条件下。我们的研究结果表明，在GSA-1细胞中，自由落体加速凋亡，这一过程与p53和Bax的积累，以及CPD的去除。因此，GSA-1细胞应该是有用的调查机制的细胞反应，包括诱导细胞凋亡的重力变化的压力。
The induction of apoptosis by microgravity and/or gravity-changing stress is considered to be one of the important causes of cell death, although the molecular mechanisms of the apoptotic event remain unclarified. In this study, we established a cell line, GSA-1, from ethyl methanesulfonate-treated human RSa cells. GSA-1 cells were highly susceptible to apoptosis after a free-fall; 24.4% of these cells underwent apoptosis after free-fall, compared with only 6% of the RSa cells. The apoptosis of GSA-1 cells was augmented by ultraviolet (UV, principally 254-nm wavelength) irradiation before free-fall to a greater extents than those in RSa cells. The molecular mechanisms of apoptosis included p53 and Bax proteins; the expression of nuclear p53 and cytoplasmic Bax in GSA-1 cells increased at 4 h after free-fall irrespective of irradiation. In addition, the rate of removal of cyclobutane pyrimidine dimer (CPD) in UV-irradiated GSA-1 cells was higher in cells exposed to free-fall than in those under the 1-G condition. Our results suggested that in GSA-1 cells, free-fall accelerates apoptosis, and that this process is associated with the accumulation of p53 and Bax, as well as CPD removal. Thus, GSA-1 cells should be useful for investigating the mechanism of cellular response, including the induction of apoptosis under gravity-changing stress.
DOI：
10.1073/pnas.170282597
发表时间：
2000-08-15
影响因子：
11.1
作者：
Lin, K;Hsu, PP;Chien, S
通讯作者：
Chien, S