ACTIVATION OF MAMMALIAN OOCYTES BY INTRACELLULAR INJECTION OF CALCIUM
ACTIVATION OF MAMMALIAN OOCYTES BY INTRACELLULAR INJECTION OF CALCIUM
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DOI：
10.1038/273149a0
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发表时间：
1978-01-01
期刊：
影响因子：
64.8
通讯作者：
WHITTINGHAM, DG
中科院分区：
文献类型：
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作者：
FULTON, BP;WHITTINGHAM, DG
许多物种的卵母细胞在暴露于钙离子载体A23187后经历孤雌激活，即使在没有外部Ca 2+的情况下（参考文献1和2），而小鼠、大鼠和仓鼠的卵母细胞仅在暴露于无Ca 2+培养基时激活3，4。这表明Ca ~（2+）参与了孤雌激活。利用发光蛋白水母发光蛋白，已经获得了鱼类5和海胆卵6受精或激活时细胞内游离Ca 2+瞬时增加的直接证据。我们在这里报告，孤雌激活和随后的正常发展，可以诱导小鼠卵母细胞注射Ca 2+。
OOCYTES of many species undergo parthenogenetic activation after exposure to the calcium ionophore, A23187, even in the absence of external Ca2+(refs 1 and 2), whereas those of mouse, rat and hamster will activate simply on exposure to Ca2+free medium3,4. This suggests that Ca2+is involved in parthenogenetic activation. Direct evidence of a transient increase in intracellular free Ca2+at the time of fertilisation or activation of fish5and sea urchin eggs6has been obtained using the photoprotein aequorin. We report here that parthenogenetic activation and subsequent normal development can be induced in mouse oocytes by injecting them with Ca2+.