Genetic polymorphisms of the HLA-DP and HLA-DQ genes could influence Hepatitis B virus infection in Yunnan population
Genetic polymorphisms of the HLA-DP and HLA-DQ genes could influence Hepatitis B virus infection in Yunnan population
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HLA-DP和HLA-DQ基因多态性影响云南人群乙型肝炎病毒感染
DOI：
10.1080/08820139.2020.1733010
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发表时间：
2020-03-14
影响因子：
2.8
通讯作者：
Zhang, A-Mei
中科院分区：
文献类型：
--
作者：
Song, Yuzhu;Xia, Tian;Zhang, A-Mei
摘要乙型肝炎是由乙肝病毒感染引起的一种流行性、传染性肝炎。在东亚人群中，单核苷酸多态与乙肝病毒感染相关，但在云南人群中尚未见报道。我们招募了493名乙肝患者和460名正常对照，对7GWASSNPs进行了基因分型，并对这些SNPs与乙肝患者生化特征的相关性进行了研究。结果显示，HLADP(rs3077、9277535和3128917)和HLADQ(rs2856718和7453920)基因的SNPs的基因型和等位基因频率与乙肝病毒感染相关。3个亚型之间的基因分型频率有显著差异。Rs3130542(人类白细胞抗原-C)基因AA在#1亚组患者中的频率显著高于其他两个亚组(#1与#2，p=0.02；#1与#3，p=0.03)。同时，rs3077、rs9277535和3128917(HLADP)等位基因频率在亚组2和亚组3之间差异有统计学意义。rs3077基因携带者的间接胆红素水平显著低于CC基因携带者(p=0.009)和TT基因携带者(p=0.016)，rs3128917基因携带者的间接胆红素水平也低于GG基因携带者(p=0.015)。Rs4821116(UBE2L3)基因TT携带者的直接胆红素水平高于CT携带者(P=0.010)。综上所述，我们确定了GWASSNPs与云南地区乙肝病毒感染或生化特征之间的关系。
ABSTRACT Hepatitis B, caused by hepatitis B virus (HBV) infection, is one of the epidemic and infectious hepatitis diseases. The sigle-nucleotide polymorphisms were identified to associate with HBV infection in East Asian population by genome-wide association study (GWAS), but no study in Yunnan HBV population was reported. We recruited 493 HBV patients and 460 general controls to genotype 7 GWAS SNPs, and then, the association study was performed between these SNPs and biochemical features of HBV patients. The results showed that genotype and allele frequencies of SNPs in the HLA-DP (rs3077, 9277535, and 3128917) and HLA-DQ (rs2856718 and 7453920) genes were associated with HBV infection. Significantly different genotyping frequencies were investigated among three HBV subgroups. Genotype AA of rs3130542 (HLA-C) showed significantly higher frequency in subgroup #1 patients than the other two subgroups (#1 vs. #2, p = .02; #1 vs. #3, p = .03). Meanwhile, genotype frequencies of rs3077, rs9277535, and 3128917 (HLA-DP) were significantly different between patients in subgroup #2 and #3. The indirect bilirubin level was significantly lower in patients with genotype CT of rs3077 than patients with genotype CC (p = .009) or TT (p = .016), and it also showed lower level in patients with genotype GT of rs3128917 than patients with genotype GG (p = .015). The direct bilirubin level was higher in patients with genotype TT of rs4821116 (UBE2L3) than patients with genotype CT (p = .010). In summary, we identified the association between GWAS SNPs and HBV infection or biochemical features in Yunnan HBV population.