Magnetic mesoporous nanospheres anchored with LyP-1 as an efficient pancreatic cancer probe
Magnetic mesoporous nanospheres anchored with LyP-1 as an efficient pancreatic cancer probe
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LyP-1 锚定的磁性介孔纳米球作为有效的胰腺癌探针
DOI：
10.1016/j.biomaterials.2016.11.006
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发表时间：
2017-01-01
期刊：
影响因子：
14
通讯作者：
Zhao, Dongyuan
中科院分区：
文献类型：
--
作者：
Jiang, Yongjian;Liu, Shaojun;Zhao, Dongyuan
将与癌细胞选择性相互作用的配体固定到纳米材料上可以提高其诊断和治疗效率。在本研究中，我们首次证实了在小鼠和人胰腺癌中高表达的环状九氨基酸肽LyP-1（Cys-Gly-Asn-Lys-Arg-Thr-Arg-Gly-Cys）受体。在此基础上，合理设计了亚50 nm多功能超顺磁性介孔纳米微球，并对其表面进行了LyP-1修饰。这些纳米球具有二氧化硅保护的磁铁矿纳米颗粒的核和FITC标记的介孔二氧化硅的壳，并且它们能够特异性地识别胰腺癌细胞并与胰腺癌细胞结合，如通过荧光成像和T2加权磁共振成像的组合技术所验证的。在系统给药后，发现这些LyP-1固定的纳米球主动靶向胰腺癌的小鼠原位异种移植物，这为通过多模态成像进行胰腺癌的早期探测和诊断打开了大门。(C)2016爱思唯尔有限公司版权所有
Immobilization of a ligand that selectively interacts with cancer cells to nanomaterials can enhance their diagnostic and therapeutic efficiency. In this study, we firstly demonstrate the high expression of receptor for cyclic nine-amino acid peptide LyP-1 (Cys-Gly-Asn-Lys-Arg-Thr-Arg-Gly-Cys) in both mouse and human pancreatic cancer. Based on these findings, sub-50 nm multifunctional superparamagnetic mesoporous nanospheres with surface modified with LyP-1 are rationally designed. Theses nanospheres have a core of silica-protected magnetite nanoparticle and a shell of FITC-labeled mesoporous silica, and they are able to specifically recognize and conjugate with the pancreatic cancer cell in vitro, as verified by the combined techniques of fluorescent imaging and T2 weight magnetic resonance imaging. After systematic administration, these LyP-1 immobilized nanospheres are found to actively target to mouse orthotopic xenograft of pancreatic cancer, which opens up the door for applications in early probing and diagnosis of pancreatic cancer by the multimodal imaging. (C) 2016 Elsevier Ltd. All rights reserved.