Optimisation of insect cell growth in deep-well blocks: development of a high-throughput insect cell expression screen
Optimisation of insect cell growth in deep-well blocks: development of a high-throughput insect cell expression screen
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DOI：
10.1016/j.pep.2004.08.020
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发表时间：
2005-01-01
影响因子：
1.6
通讯作者：
Hunt, I
中科院分区：
文献类型：
--
作者：
Bahia, D;Cheung, R;Hunt, I
本报告描述了一种在24个深孔块中培养昆虫细胞的方法，用于杆状病毒介导的蛋白质表达实验的常规小规模优化。该过程的小型化提供了在资源分配、试剂和劳动力方面的必要减少，以允许表达条件的广泛和快速优化。伴随着在大规模生物反应器实验开始之前的前置时间的减少。因此，这大大简化了优化过程，并允许在该过程的大部分初始优化阶段使用液体处理机器人。从而大大增加了实验室的生产能力。我们提出了几个使用深井块表达研究的例子，在治疗相关的蛋白质靶点的优化。我们还讨论了如何提高这种方法提供的吞吐量可以适应机器人处理系统和这对进行多并行蛋白质表达研究的能力的影响。(C)2004爱思唯尔公司All rights reserved.
This report describes a method to culture insects cells in 24 deep-well blocks for the routine small-scale optimissation of baculovirus-mediated protein expression experiments. Miniaturisation of this process provides the necessary reduction in terms of resource allocation, reagents, and labour to allow extensive and rapid optimisation of expression conditions. with the concomitant reduction in lead-time before commencement of large-scale bioreactor experiments. This therefore greatly simplifies the optimisation process and allows the use of liquid handling robotics in much of the initial optimisation stages of the process. thereby greatly increasing the throughput of the laboratory. We present several examples of the use of deep-well block expression studies in the optimisation of therapeutically relevant protein targets. We also discuss how the enhanced throughput offered by this approach can be adapted to robotic handling systems and the implications this has on the capacity to conduct multi-parallel protein expression studies. (C) 2004 Elsevier Inc. All rights reserved.