Electrophoretic Mobility Shift Assay (EMSA) and Supershift Assay of Cytochrome P450 2B6 in Response to Estrogen.
Electrophoretic Mobility Shift Assay (EMSA) and Supershift Assay of Cytochrome P450 2B6 in Response to Estrogen.
复制标题
细胞色素 P450 2B6 对雌激素反应的电泳迁移率变化测定 (EMSA) 和 Supershift 测定。
DOI：
10.1007/978-1-4939-3127-9_5
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Jeong,Hyunyoung
中科院分区：
文献类型：
--
作者：
Koh,KwiHye;Jeong,Hyunyoung
Electrophoretic mobility shift assay (EMSA) is an invaluable tool to study interaction of proteins with DNA. Estrogens are major female hormones and modulate biological function through estrogen receptor (ER). ER regulates its target gene expression via the classical mechanism in which ER directly binds to its target gene promoter or the nonclassical mechanism involving tethering of ER to other transcription factors (such as AP-1 proteins). Here, we describe the EMSA to examine the nonclassical mechanism of ER action in regulation of a gene CYP2B6 by using competition and supershift assays.
