CADMIUM ACCELERATES BONE LOSS IN OVARIECTOMIZED MICE AND FETAL-RAT LIMB BONES IN CULTURE
CADMIUM ACCELERATES BONE LOSS IN OVARIECTOMIZED MICE AND FETAL-RAT LIMB BONES IN CULTURE
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DOI：
10.1073/pnas.85.22.8761
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发表时间：
1988-11-01
影响因子：
11.1
通讯作者：
PETERSON, DP
中科院分区：
文献类型：
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作者：
BHATTACHARYYA, MH;WHELTON, BD;PETERSON, DP
研究了暴露于0.25、5或50ppm膳食镉的小鼠卵巢切除后骨矿物质的丢失。结果与假手术对照组相比，饮食中50ppm的镉显著增加了去卵巢小鼠的骨矿物质丢失。这些结果来自两项研究，一项是在卵巢切除6个月后测定骨骼钙含量，另一项是在开始饮食镉暴露后立即测量45Ca预标记骨的45Ca释放量。此外，用45Ca标记的胎鼠四肢骨在培养中的实验表明，在10 NM的培养液中，Cd的浓度估计在暴露于50ppm膳食Cd的小鼠的血浆中，显著增加骨吸收，从27.+-。2%(平均+-扫描电子显微镜)~(45)Ca在不加镉的培养体系中释放到68~+-。含镉培养物释放6%(n=4)。这些体外结果表明，镉可能通过直接作用于骨骼而增加骨矿物质丢失。活体研究的结果与镉在日本绝经后女性Itai-Itai病的病因中扮演的重要角色一致，也可能在一定程度上解释了吸烟女性患绝经后骨质疏松症风险增加的原因。
Loss of bone mineral after ovariectomy was studied in mice exposed to dietary cadmium at 0.25, 5, or 50 ppm. Results that dietary cadmium at 50 ppm increased bone mineral loss to a significantly greater extent in ovariectomized mice than in sham-operated controls. These results were obtained from two studies, one in which skeletal calcium content was determined 6 months after ovariectomy and a second in which 45Ca release from 45Ca-prelabeled bones was measured immediately after the start of dietary cadmium exposure. Furthermore, experiments with 45Ca-prelabeled fetal rat limb bones in culture demonstrated that Cd at 10 nM in the medium, a concentration estimated to be in the plasma of mice exposed to 50 ppm dietary Cd, strikingly increased bone resorption, from 27 .+-. 2% (mean .+-. SEM) 45Ca release in cultures with no added cadmium to 68 .+-. 6% release in cultures containing cadmium (n = 4). These in vitro results indicate that cadmium may enhance bone mineral loss by a direct action on bone. Results of the in vivo studies are consistent with a significant role of cadmium in the etiology of Itai-Itai disease among postmenopausal women in Japan and may in part explain the increased risk of postmenopausal osteoporosis among women who smoke.