Sensitive and selective detection of iron-catalysed hydroxyl radical formation in water without chemical probes
Sensitive and selective detection of iron-catalysed hydroxyl radical formation in water without chemical probes
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DOI：
10.1039/c3ay26256a
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发表时间：
2013-05
影响因子：
3.1
通讯作者：
Wei-Chung Chen;Jen-Lin Chang;J. Zen
中科院分区：
文献类型：
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作者：
Wei-Chung Chen;Jen-Lin Chang;J. Zen
我们报道了一种不需要任何分离过程和化学探针，通过耗尽H_2O_2作为铁催化的羟基自由基形成的指示剂的高选择性羟基自由基检测方法。在合适的湿度下用氮气吹扫，过氧化氢可以在气体介质中用水进行检测，并使用由沉积有铂纳米颗粒的丝网印刷边缘带碳超微电极和涂有Nafion的一次性传感器作为固体聚合物电解质。该H_2O_2检测方案具有很高的选择性，无需任何分离过程和化学探针即可监测羟基自由基。在Fenton化学的基础上，通过抑制过氧化氢的峰电流实现了羟基自由基的定量，在25μM~1 mm范围内与[Fe2+]呈线性关系，检出限为4.48μM(S/N=3)。该方法已被用于评价酚类化合物和儿茶素类化合物的抗氧化活性。
We report here a highly selective hydroxyl radical assay through the depletion of H2O2 as an indicator for iron-catalysed hydroxyl radical formation without any separation process and chemical probe. By purging with N2 gas under suitable humidity, H2O2 can be carried out with water and detected in the gas media using a disposable sensor made of a screen-printed edge band carbon ultramicroelectrode deposited with Pt nanoparticles and coated with Nafion as the solid polymer electrolyte. This H2O2 detection scheme is highly selective and thus can monitor the hydroxyl radical without any separation process and chemical probe. Based on Fenton chemistry, the quantitation of the hydroxyl radical is achieved through the suppressed peak current of hydrogen peroxide with a linear dependence on [Fe2+] in the range of 25 μM to 1 mM and a detection limit of 4.48 μM (S/N = 3). The proposed method has been applied to evaluate the antioxidative activity of phenolic compounds and catechins.