Genetic analysis of Netrin genes in Drosophila: Netrins guide CNS commissural axons and peripheral motor axons
Genetic analysis of Netrin genes in Drosophila: Netrins guide CNS commissural axons and peripheral motor axons
复制标题
DOI：
10.1016/s0896-6273(00)80153-1
复制
发表时间：
1996-08-01
期刊：
影响因子：
16.2
通讯作者：
Dickson, BJ
中科院分区：
文献类型：
--
作者：
Mitchell, KJ;Doyle, JL;Dickson, BJ
果蝇中的两个串联 Netrin 基因在发育中的中枢神经系统的中线以及不同的神经元、肌肉和表皮斑块中表达。在携带两个基因缺失的胚胎中，中枢神经系统轴突连合部分缺失或变薄。通过在中线表达任一 Netrin 基因可以挽救这种表型。任一基因的全神经表达都会导致连合束和纵束的破坏，表明 Netrin 表达模式至关重要，并且 Netrin 可以起到指导性线索的作用。双突变体还显示出运动轴突投射的缺陷。 Netrin 基因在所有肌肉中的表达也会导致异常的运动投射。因此，果蝇 Netrins 是引导中线连合轴突以及引导运动轴突到达目标肌肉所必需的。
Two tandem Netrin genes in Drosophila are expressed at the midline of the developing CNS and in different subsets of neurons, muscles, and epidermal patches. In embryos carrying a small deficiency that deletes both genes, CNS axon commissures are partially missing or thinner. This phenotype is rescued by expressing either Netrin gene at the midline. Pan-neural expression of either gene causes disruption of commissural and longitudinal tracts, indicating that the pattern of Netrin expression is crucial and that Netrins function as instructive cues. The double mutant also shows defects in motor axon projections. Expression of either Netrin gene in all muscles also results in aberrant motor projections. Thus, Drosophila Netrins are required for the guidance of commissural axons at the midline, and of motor axons to their target muscles.