Trypanosoma cruzi IV causing outbreaks of acute Chagas disease and infections by different haplotypes in the Western Brazilian Amazonia.
Trypanosoma cruzi IV causing outbreaks of acute Chagas disease and infections by different haplotypes in the Western Brazilian Amazonia.
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DOI：
10.1371/journal.pone.0041284
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Barbosa Md
中科院分区：
文献类型：
--
作者：
Monteiro WM;Magalhães LK;de Sá AR;Gomes ML;Toledo MJ;Borges L;Pires I;Guerra JA;Silveira H;Barbosa Md
查加斯病是巴西亚马逊地区的一种紧急热带疾病，近几十年来病例数量不断增加。在这一地区，锥虫克氏锥虫传播，这构成了一个水库的寄生虫，可能与特定的分子，流行病学和临床特征，一直很少探讨。这项工作的目的是遗传特性的股票T。在巴西亚马逊西部的亚马逊州，从人类病例、锥蝽和水库哺乳动物中发现了克鲁齐氏病。我们分析了96个T. cruzi样本来自亚马逊州偏远地区的四个城市。通过对小外显子和24 S α核糖体RNA基因的非转录间隔区进行PCR，对线粒体细胞色素c氧化酶亚基II（COII）基因进行RFLP和测序，以及对葡萄糖磷酸异构酶基因进行测序，对LIT培养基中培养物或直接从载体或全血中分离的寄生虫进行分子表征。霸王来自两次急性疾病爆发的克氏寄生虫均被分型为TcIV。其中一次爆发是由同一DTU的几个单倍型引发的。TcIV也发生在孤立的情况下，并在Rhodnius robustus。线粒体和细胞核同源性之间的不一致可能是历史遗传交换事件的指示，导致从巴西亚马逊西部的TcIII和TcIV DTU之间的线粒体渐渗。TcI在锥蝽中占主导地位，是感染有袋类动物的唯一DTU。DTU TcIV很少与亚马逊盆地其他地区的人类恰加斯病相关，是巴西亚马逊西部人类感染的主要菌株，在爆发中以不同单倍型的单一或混合感染发生。
Chagas disease is an emergent tropical disease in the Brazilian Amazon Region, with an increasing number of cases in recent decades. In this region, the sylvatic cycle of Trypanosoma cruzi transmission, which constitutes a reservoir of parasites that might be associated with specific molecular, epidemiological and clinical traits, has been little explored. The objective of this work is to genetically characterize stocks of T. cruzi from human cases, triatomines and reservoir mammals in the State of Amazonas, in the Western Brazilian Amazon. We analyzed 96 T. cruzi samples from four municipalities in distant locations of the State of Amazonas. Molecular characterization of isolated parasites from cultures in LIT medium or directly from vectors or whole human blood was performed by PCR of the non-transcribed spacer of the mini-exon and of the 24 S alfa ribosomal RNA gene, RFLP and sequencing of the mitochondrial cytochrome c oxidase subunit II (COII) gene, and by sequencing of the glucose-phosphate isomerase gene. The T. cruzi parasites from two outbreaks of acute disease were all typed as TcIV. One of the outbreaks was triggered by several haplotypes of the same DTU. TcIV also occurred in isolated cases and in Rhodnius robustus. Incongruence between mitochondrial and nuclear phylogenies is likely to be indicative of historical genetic exchange events resulting in mitochondrial introgression between TcIII and TcIV DTUs from Western Brazilian Amazon. TcI predominated among triatomines and was the unique DTU infecting marsupials. DTU TcIV, rarely associated with human Chagas disease in other areas of the Amazon basin, is the major strain responsible for the human infections in the Western Brazilian Amazon, occurring in outbreaks as single or mixed infections by different haplotypes.
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影响因子：
2
作者：
Florez, Oscar;Esper, Jhonatan;Gonzalez Rugeles, Clara Isabel
通讯作者：
Gonzalez Rugeles, Clara Isabel
影响因子：
4
作者：
Brisse, S;Verhoef, J;Tibayrenc, M
通讯作者：
Tibayrenc, M
影响因子：
64.8
作者：
Gaunt, MW;Yeo, M;Miles, MA
通讯作者：
Miles, MA
DOI：
10.1590/s0074-02762007005000098
发表时间：
2007-10-01
期刊：
Memórias do Instituto Oswaldo Cruz
影响因子：
--
作者：
Aguilar, Hugo Marcelo;Abad-Franch, Fernando;Coura, José Rodrigues
通讯作者：
Coura, José Rodrigues
DOI：
10.1590/s0037-86822008000500020
发表时间：
2008-10-01
影响因子：
2
作者：
Medeiros, Maurício Borborema de;Guerra, Jorge Augusto de Oliveira;Lacerda, Marcus Vinícius Guimarães de
通讯作者：
Lacerda, Marcus Vinícius Guimarães de