A genome-wide association study for racing performances in Thoroughbreds clarifies a candidate region near the MSTN gene
A genome-wide association study for racing performances in Thoroughbreds clarifies a candidate region near the MSTN gene
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DOI：
10.1111/j.1365-2052.2010.02095.x
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发表时间：
2010-12-01
期刊：
影响因子：
2.4
通讯作者：
Nakano, Y.
中科院分区：
文献类型：
--
作者：
Tozaki, T.;Miyake, T.;Nakano, Y.
P>使用 1400 个微卫星，进行了全基因组关联研究 (GWAS)，以确定与日本竞马协会 (JRA) 确定的终生收入和表现排名相关的基因组区域。根据数量性状模型估计最小遗传力（h2）为7-8%，表明赛车成绩是可遗传的。利用微卫星进行 GWAS 后，精细作图识别了 ECA18 上的三个 SNP，即与这些性能测量相关的 g.65809482T > C (P = 1.05E-18)、g.65868604G > T (P = 6.47E-17) 和 g.66539967A > G (P = 3.35E-14)。这些 SNP 的单倍型以及最近发表的附近 SNP g.66493737C > T (P = 9.06E-16) 处于强连锁不平衡状态，也显示出与性能的非常明显的关联 (P < 1E-05)。候选基因组区域包含 ENSEMBL 注释的 8 个基因，包括肌肉生长抑制素基因 (MSTN)。这些发现表明，在 ECA18 上存在影响该地区纯种赛马比赛表现的基因。
P>Using 1400 microsatellites, a genome-wide association study (GWAS) was performed to identify genomic regions associated with lifetime earnings and performance ranks, as determined by the Japan Racing Association (JRA). The minimum heritability (h2) was estimated at 7-8% based on the quantitative trait model, suggesting that the racing performance is heritable. Following GWAS with microsatellites, fine mapping led to identification of three SNPs on ECA18, namely, g.65809482T > C (P = 1.05E-18), g.65868604G > T (P = 6.47E-17), and g.66539967A > G (P = 3.35E-14) associated with these performance measures. The haplotype of these SNPs, together with a recently published nearby SNP, g.66493737C > T (P = 9.06E-16) in strong linkage disequilibrium, also showed a very clear association with the performance (P < 1E-05). The candidate genomic region contained eight genes annotated by ENSEMBL, including the myostatin gene (MSTN). These findings suggest the presence of a gene affecting the racing performance in Thoroughbred racehorses in this region on ECA18.