Butanol production from wheat straw hydrolysate using Clostridium beijerinckii
Butanol production from wheat straw hydrolysate using Clostridium beijerinckii
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DOI：
10.1007/s00449-007-0137-9
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发表时间：
2007-11-01
影响因子：
3.8
通讯作者：
Cotta, Michael A.
中科院分区：
文献类型：
--
作者：
Qureshi, Nasib;Saha, Badal C.;Cotta, Michael A.
在这些研究中，利用贝氏梭菌P260从小麦秸秆水解物（WSH）中批量培养产生丁醇（丙酮丁醇乙醇或ABE）。对照发酵时，以48.9 g L-1葡萄糖（初始糖62.0 g L-1）产ABE 20.1 g L-1，产率和产率分别为0.28 g L(-1)h(-1) stop和0.41 g L-1。在一个类似的实验中，使用WSH（由86 g L-1麦秸水解得到的60.2 g L-1总糖），培养物产生25.0 g L-1 ABE，生产力和产量分别为0.60 g L(-1)h（-1）和0.42。结果优于对照试验，产量提高214%。当WSH中添加35 g L-1葡萄糖时，反应器生产率提高到0.63 g L(-1)h(-1)，产率为0.42。在这种情况下，肉汤中ABE的浓度为28.2 g L-1。当WSH中添加60 g L-1葡萄糖时，含有128.3 g L-1糖的培养基成功发酵（由于产物去除），产生47.6 g L(-1)p ABE，培养物利用了所有糖（葡萄糖、木糖、阿拉伯糖、半乳糖和甘露糖）。这些结果表明，C. beijerinckii P260具有将生物质衍生糖转化为溶剂的良好能力，可以从水sh中产生超过28 g L-1的ABE（在一个案例中，葡萄糖为41.7 g L-1）。含有250 g L-1葡萄糖的培养基没有生长，也没有产生ABE。含有水sh + 140 g L-1葡萄糖（总糖约为200 g L-1）的混合物表现出生长不良和ABE产生不良。
In these studies, butanol (acetone butanol ethanol or ABE) was produced from wheat straw hydrolysate (WSH) in batch cultures using Clostridium beijerinckii P260. In control fermentation 48.9 g L-1 glucose (initial sugar 62.0 g L-1) was used to produce 20.1 g L-1 ABE with a productivity and yield of 0.28 g L(-1)h(-1) stop and 0.41, respectively. In a similar experiment where WSH (60.2 g L-1 total sugars obtained from hydrolysis of 86 g L-1 wheat straw) was used, the culture produced 25.0 g L-1 ABE with a productivity and yield of 0.60 g L(-1)h(-1) and 0.42, respectively. These results are superior to the control experiment and productivity was improved by 214%. When WSH was supplemented with 35 g L-1 glucose, a reactor productivity was improved to 0.63 g L(-1)h(-1) with a yield of 0.42. In this case, ABE concentration in the broth was 28.2 g L-1. When WSH was supplemented with 60 g L-1 glucose, the resultant medium containing 128.3 g L-1 sugars was successfully fermented (due to product removal) to produce 47.6 g L(-1)p ABE, and the culture utilized all the sugars (glucose, xylose, arabinose, galactose, and mannose). These results demonstrate that C. beijerinckii P260 has excellent capacity to convert biomass derived sugars to solvents and can produce over 28 g L-1 (in one case 41.7 g L-1 from glucose) ABE from WSH. Medium containing 250 g L-1 glucose resulted in no growth and no ABE production. Mixtures containing WSH + 140 g L-1 glucose (total sugar approximately 200 g L-1) showed poor growth and poor ABE production.