THE ROLE OF IRON IMPURITIES IN THE TOXIC EFFECTS EXERTED BY SHORT MULTIWALLED CARBON NANOTUBES (MWCNT) IN MURINE ALVEOLAR MACROPHAGES
THE ROLE OF IRON IMPURITIES IN THE TOXIC EFFECTS EXERTED BY SHORT MULTIWALLED CARBON NANOTUBES (MWCNT) IN MURINE ALVEOLAR MACROPHAGES
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DOI：
10.1080/15287394.2013.834855
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发表时间：
2013-09-17
影响因子：
2.6
通讯作者：
Fubini, B.
中科院分区：
文献类型：
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作者：
Aldieri, E.;Fenoglio, I.;Fubini, B.
多壁碳纳米管(MWCNT)介导的肺毒性已被广泛证实，并与诱发致癌石棉样效应有关，但这种毒性效应的化学特征仍未被很好地阐明。在MWCNT制备过程中，金属作为微量污染物的存在，特别是铁(Fe)杂质的存在，在决定细胞对MWCNT的不同反应中发挥了重要作用。我们的目标是通过将两个不同的MWCNT样品暴露于两个不同的MWCNT样品中，阐明MWCNT诱导毒性的机制与铁杂质的存在相关。数据显示，只有富铁的MWCNT具有显著的细胞毒性和遗传毒性，并诱导强烈的细胞氧化应激，而不含Fe的MWCNT没有产生任何这些不利影响。这些结果证实，铁含量是促进多壁碳纳米管毒性的重要关键成分，在规划新的、更安全的制备路线时，需要考虑这一点。
Lung toxicity mediated by multiwalled carbon nanotubes (MWCNT) has been widely demonstrated and recently associated with induction of carcinogenic asbestos-like effects, but the chemical features that drive this toxic effect have still not been well elucidated. The presence of metals as trace contaminants during MWCNT preparation, in particular iron (Fe) impurities, plays an important role in determining a different cellular response to MWCNT. Our goal was to clarify the mechanisms underlying MWCNT-induced toxicity with correlation to the presence of Fe impurities by exposing murine alveolar macrophages to two different MWCNT samples, which differed only in the presence or absence of Fe. Data showed that only Fe-rich MWCNT were significantly cytotoxic and genotoxic and induced a potent cellular oxidative stress, while Fe-free MWCNT did not exert any of these adverse effects. These results confirm that Fe content represents an important key constituent in promoting MWCNT-induced toxicity, and this needs to be taken into consideration when planning new, safer preparation routes.