Characterization of the active fragments of Spodoptera litura Lebocin-1
Characterization of the active fragments of Spodoptera litura Lebocin-1
复制标题
斜纹夜蛾 Lebocin-1 活性片段的表征
DOI：
10.1002/arch.21626
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发表时间：
2020
影响因子：
2.2
通讯作者：
Xiang‐Jun Rao
中科院分区：
文献类型：
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作者：
Li‐Ling Yang;Ming‐Yue Zhan;Yu‐Li Zhuo;Xiang‐Li Dang;Mao‐Ye Li;Yang Xu;Xiu‐Hong Zhou;Xiao‐Qiang Yu;Xiang‐Jun Rao
昆虫在免疫刺激下可产生多种抗菌肽（AMPs）。一类amp的特点是在某些片段中脯氨酸含量高。它们通常被称为富含脯氨酸的抗菌肽（pramp）。我们之前报道了一种PrAMP蛋白spodoptera lituralebocin‐1 （SlLeb‐1）的特性。对合成多肽的初步研究表明，在4个演绎活性片段中，C端片段SlLeb‐1（124‐158）表现出较强的抗菌活性。在此，我们进一步鉴定了124‐158及其四个亚片段：124‐155、124‐149、127‐158和135‐158的抗菌和抗真菌活性。只有124‐158和127‐158能凝集细菌，而124‐158和4个亚片段都能凝集球孢白僵菌。共聚焦显微镜显示，荧光肽位于微生物表面。片段135‐158完全丧失了对大肠杆菌和金黄色葡萄球菌的活性，部分丧失了对枯草芽孢杆菌的活性。只有124‐149对粘质沙雷氏菌表现出低活性。124 - 158处理过的细菌的阴性染色、透射电镜和扫描电镜显示出不同的形态。流式细胞术分析s。金黄色葡萄球菌表明，124‐158和4个亚片段改变了细菌亚群，并导致DNA含量增加。这些结果表明SlLeb‐1的活性片段可能对不同的微生物具有不同的抗菌作用。本研究为新型抗菌药物的开发提供了新的思路。
Insects can produce various antimicrobial peptides (AMPs) upon immune stimulation. One class of AMPs are characterized by their high proline content in certain fragments. They are generally called proline‐rich antimicrobial peptides (PrAMPs). We previously reported the characterization ofSpodoptera lituralebocin‐1 (SlLeb‐1), a PrAMP proprotein. Preliminary studies with synthetic polypeptides showed that among the four deductive active fragments, the C‐terminal fragment SlLeb‐1 (124‐158) showed strong antibacterial activities. Here, we further characterized the antibacterial and antifungal activities of 124‐158 and its four subfragments: 124‐155, 124‐149, 127‐158, and 135‐158. Only 124‐158 and 127‐158 could agglutinate bacteria, while 124‐158 and four subfragments all could agglutinateBeauveria bassianaspores. Confocal microscopy showed that fluorescent peptides were located on the microbial surface. Fragment 135‐158 lost activity completely againstEscherichia coliandStaphylococcus aureus, and partially againstBacillus subtilis. Only 124‐149 showed low activity againstSerratia marcescens. Negative staining, transmission, and scanning electron microscopy of 124‐158 treated bacteria showed different morphologies. Flow cytometry analysis ofS. aureusshowed that 124‐158 and four subfragments changed bacterial subpopulations and caused an increase of DNA content. These results indicate that active fragments of SlLeb‐1 may have diverse antimicrobial effects against different microbes. This study may provide an insight into the development of novel antimicrobial agents.