Immunogenicity of Vibrio cholerae O1 toxin-coregulated pili in experimental and clinical cholera.
Immunogenicity of Vibrio cholerae O1 toxin-coregulated pili in experimental and clinical cholera.
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霍乱弧菌 O1 毒素协同调节菌毛在实验和临床霍乱中的免疫原性。
DOI：
10.1128/iai.59.7.2508-2512.1991
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发表时间：
1991
影响因子：
3.1
通讯作者：
Levine,MM
中科院分区：
文献类型：
--
作者：
Hall,RH;Losonsky,G;Silveira,AP;Taylor,RK;Mekalanos,JJ;Witham,ND;Levine,MM
编码毒素共调节菌毛 (TCP) 主要亚基的功能性 tcpA 基因对于霍乱弧菌 O1 小川菌株 395 定植于人体肠道并赋予对毒性攻击的保护性免疫力是必需的。测定了 TCP 和其他抗原在实验性霍乱和自然获得性霍乱中的免疫原性。在志愿者中发现了霍乱毒素 (CT)、全细胞制剂和小川脂多糖的血清转化，但未发现纯化的天然 TCP 或 TcpA 模拟表位。志愿者的局部肠道分泌型免疫球蛋白 A 转化为霍乱毒素和脂多糖，但未转化为 TCP。印度尼西亚的霍乱患者表现出 6 分之 3 的 TCP 血清转化率和 6 分之 1 的 TcpA 模拟表位血清转化率。当针对 TCP 阳性和 TCP 阴性霍乱弧菌 O1 菌株进行检测时，志愿者和患者血清显示出相似的杀弧菌血清转化，表明 TCP 对杀弧菌没有明显贡献 抗原。我们的结论是，虽然自然获得性霍乱中可能发生 TCP 血清转化，但在缺乏可检测到的抗 TCP 免疫反应的情况下，也可以产生可靠的长期保护。
A functional tcpA gene, encoding the major subunit of toxin-coregulated pili (TCP), is necessary for Vibrio cholerae O1 Ogawa strain 395 to colonize the human intestine and confer protective immunity to virulent challenge. The immunogenicity of TCP and other antigens in experimental and naturally acquired cholera was determined. Seroconversion to cholera toxin (CT), whole cell preparations, and to Ogawa lipopolysaccharide but not to purified native TCP or to a TcpA mimiotope was found in volunteers. Local intestinal secretory immunoglobulin A from volunteers showed conversions to cholera toxin and lipopolysaccharide but not to TCP. Cholera patients in Indonesia showed a seroconversion rate to TCP of 3 of 6 and a seroconversion to a TcpA mimiotope of 1 of 6. Volunteer and patient sera showed similar vibriocidal seroconversions when assayed against either TCP-positive and TCP-negative V. cholerae O1 strains, suggesting that TCP do not contribute demonstrably to the vibriocidal antigen. We conclude that although seroconversion to TCP can occur in naturally acquired cholera, solid long-term protection can be engendered in the absence of a detectable anti-TCP immune response.