Intermittent inhalation of nitrogen dioxide: effects on rabbit alveolar macrophages.
Intermittent inhalation of nitrogen dioxide: effects on rabbit alveolar macrophages.
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间歇性吸入二氧化氮：对兔肺泡巨噬细胞的影响。
DOI：
10.1080/15287398709531007
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Schlesinger,RB
中科院分区：
文献类型：
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作者：
Schlesinger,RB
兔子每天暴露于 0.3 或 1 ppm NO2 下 2 小时，并评估暴露系列期间 3 个时间点对通过支气管肺灌洗恢复的肺泡巨噬细胞的影响。在 2、6 或 13 次暴露后 24 小时（即第 3、7 和 14 天）检查细胞。在任何时间恢复的巨噬细胞的活力或数量都没有变化，也没有产生炎症反应。巨噬细胞附着在表面的能力不受暴露的影响。在 0.3 ppm 暴露系列中，随机迁移率在第 3 天降低，但在任何 1 ppm 暴露后与对照没有不同。吞噬指数，即能够吞噬乳胶颗粒的活细胞分数，不受 NO2 暴露的影响。然而，在两种 NO2 水平下，吞噬能力（即内化特定数量颗粒的吞噬细胞数量）与第 3 天的对照不同。在 0.3 ppm 时，颗粒吸收减少，而在 1 ppm 时，颗粒吸收增加，因为更大比例的细胞含有大量乳胶颗粒。结果表明，吞噬能力和随机迁移率是对 NO2 反应的敏感指标，并且显然发生了对暴露的适应，因为吞噬作用和迁移率在第 7 d 时恢复到控制水平，并在整个暴露系列的持续时间内保持在该水平。
Rabbits were exposed to 0.3 or 1 ppm NO2for 2 h/d and the effects on alveolar macrophages recovered by bronchopulmonary lavage at 3 time points during the exposure series were asessed. Cells were examined 24 h after 2, 6, or 13 exposures, i.e., on d 3, 7, and 14. There was no change in the viability or numbers of macrophages recovered at any time, nor was an inflammatory response produced. The ability of macrophages to attach to a surface was not affected by exposure. Random mobility was reduced at d 3 in the 0.3 ppm exposure series, but was not different from control after any of the 1 ppm exposures. The phagocytic index, i.e., the fraction of viable cells able to phagocytize latex particles, was not affected by NO2exposure. However, phagocytic capacity—i.e., the numbers of phagocytizing cells that internalized specific numbers of particles—was different from control on d 3 with both levels of NO2. At 0.3 ppm, particle uptake was reduced, while at 1 ppm it was enhanced in that a greater fraction of cells contained a large number of latex particles. The results show that phagocytic capacity and random mobility are sensitive indices of response to NO2and that adaptation to the exposures apparently occurred in that both phagocytosis and mobility returned to control levels by d 7 and remained there through the duration of the exposure series.