Carbon Nanotubes as Supports for Inulinase Immobilization
Carbon Nanotubes as Supports for Inulinase Immobilization
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DOI：
10.3390/molecules190914615
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发表时间：
2014-09-01
期刊：
影响因子：
4.6
通讯作者：
Kuhn, Raquel C.
中科院分区：
文献类型：
--
作者：
Garlet, Tais B.;Weber, Caroline T.;Kuhn, Raquel C.
从黑曲霉中获得的商业菊粉酶被非共价固定在多壁碳纳米管（MWNT-COOH）上。碳纳米管的固定条件由中心复合旋转设计（CCRD）确定。研究了酶浓度(0.8%-1.7% v/v)和吸附剂:吸附质比(1:460-1:175)对酶固定化的影响。在 90% 显着性水平上，吸附剂：吸附物比率变量对菊粉酶固定 (U/g) 响应具有正向影响，而酶浓度则具有负向影响。这些结果表明，酶浓度越低和吸附剂：吸附物比率越高，固定化效果越好。根据结果，可以观察到碳纳米管表现出有效的菊粉酶吸附作用。使用 1.3% (v/v) 菊粉酶浓度和 1:460 吸附剂：吸附物比率，观察到约六分钟内的快速吸附和 51,047 U/g 载体的负载能力。评估了温度对固定化酶活性的影响，在50 phi C下显示出更好的活性。固定化酶在室温下五周内保持了100%的活性。实验设计确定了 MWNT-COOH 的固定化策略，表明菊粉酶固定化是碳纳米管的一种有前景的生物技术应用。
The commercial inulinase obtained from Aspergillus niger was non-covalently immobilized on multiwalled carbon nanotubes (MWNT-COOH). The immobilization conditions for the carbon nanotubes were defined by the central composite rotational design (CCRD). The effects of enzyme concentration (0.8%-1.7% v/v) and adsorbent: adsorbate ratio (1:460-1:175) on the enzyme immobilization were studied. The adsorbent: adsorbate ratio variable has positive effect and the enzyme concentration has a negative effect on the inulinase immobilization (U/g) response at the 90% significance level. These results show that the lower the enzyme concentration and the higher the adsorbent: adsorbate ratio, better is the immobilization. According to the results, it is possible to observe that the carbon nanotubes present an effective inulinase adsorption. Fast adsorption in about six minutes and a loading capacity of 51,047 U/g support using a 1.3% (v/v) inulinase concentration and a 1: 460 adsorbent: adsorbate ratio was observed. The effects of temperature on the immobilized enzyme activity were evaluated, showing better activity at 50 phi C. The immobilized enzyme maintained 100% of its activity during five weeks at room temperature. The immobilization strategy with MWNT-COOH was defined by the experimental design, showing that inulinase immobilization is a promising biotechnological application of carbon nanotubes.