Video-based spatio-temporal maps for analysis of gastric motility in vitro:: effects of vagal stimulation in guinea-pigs
Video-based spatio-temporal maps for analysis of gastric motility in vitro:: effects of vagal stimulation in guinea-pigs
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DOI：
10.1046/j.1365-2982.2002.00369.x
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发表时间：
2002-12-01
影响因子：
3.5
通讯作者：
Costa, M
中科院分区：
文献类型：
--
作者：
Berthoud, HR;Hennig, G;Costa, M
我们的目的是评估由分级迷走神经激活引起的地形特异性胃运动变化。最近开发的一种从视频电影构建运动时空图的方法适用于具有完整迷走神经供应的体外灌注豚鼠胃。在未刺激的制剂中，自发活动较低或不存在。频率低至 0.2 Hz 的双侧迷走神经刺激会触发微弱的肛门，在某些情况下，会以约 5-6 分钟(-1) 的速率传播胃窦收缩。接受更高频率的刺激后，胃窦收缩的长度（从更近端开始）和幅度显着增加，并产生高达 25 hPa 的大压力峰值，在 2-4 Hz 时效果最大。相反，传播速度和蠕动波之间的间隔不随任何频率的迷走神经刺激而改变。迷走神经刺激还产生显着且与频率相关的眼底扩大，在 4 Hz 时效果最大。结论是，非常低的强直迷走神经活动对于表达基本的胃窦运动显然是必要和充分的，而更持续的迷走神经活动对于高幅度的胃收缩和显着的持续胃底松弛是必要的。传播收缩之间的恒定间隔支持这样的概念：迷走神经输入影响内在肠神经回路，其在慢波蠕动的生肌机制中具有调节作用，而不是直接影响胃肌肉组织。
Our aim was to evaluate topographically specific gastric motility changes induced by graded vagal activation. A recently developed method of constructing spatio-temporal maps of motility from video movies was adapted to the in vitro perfused guinea-pig stomach with an intact vagal nerve supply. In the unstimulated preparation, spontaneous activity was low or absent. Bilateral vagal stimulation with frequencies as low as 0.2 Hz triggered weak anally, and in some cases orally, propagating antral contractions at rates of about 5-6 min(-1). Upon stimulation with higher frequencies, antral contractions increased significantly in length ( starting more proximally) and amplitude, and produced large pressure peaks of up to 25 hPa, with maximal effects at 2-4 Hz. In contrast, the speed of propagation and the interval between peristaltic waves did not change with vagal stimulation at any frequency. Vagal stimulation also produced a significant and frequency-dependent enlargement of the fundus with a maximal effect at 4 Hz. It is concluded that a very low tonic vagal activity is apparently necessary and sufficient to express basic antral motility, while more sustained vagal activity is necessary for high-amplitude gastric contractions and significant sustained fundic relaxation. The constant interval between propagating contractions supports the concept that vagal input impinges on intrinsic enteric neural circuits that have a modulatory role in the myogenic mechanism underlying slow-wave peristalsis, rather than directly on gastric musculature.