Viable Cell Detection by the Combined Use of Fluorescent Glucose and Fluorescent Glycine

Viable Cell Detection by the Combined Use of Fluorescent Glucose and Fluorescent Glycine
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联合使用荧光葡萄糖和荧光甘氨酸检测活细胞

DOI:
10.1271/bbb.67.2459
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发表时间:
2003
期刊:
Bioscience, Biotechnology, and Biochemistry
影响因子:
--
通讯作者:
S. Igimi
S. Igimi
中科院分区:
--
文献类型:
--
作者:
H. Matsuoka;K. Oishi;Masaaki Watanabe;I. Kozone;M. Saito;S. Igimi

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除了几种大肠杆菌菌株和大肠菌群之外,还尝试联合使用荧光葡萄糖 (2NBDG) 和荧光甘氨酸 (NBD-Gly) 来检测重要食源性致病菌株的活细胞。 41株中的35株显示出对2NBDG的显着摄取,但6株不能摄取2NBDG。这 6 个菌株中有 5 个表现出 NBD-Gly 摄取。
The combined use of a fluorescent glucose (2NBDG) and a fluorescent glycine (NBD-Gly) was tried for the detection of viable cells of significant foodborne pathogenic strains in addition to several Escherichia coli strains and coliforms. Thirty-five out of 41 strains showed marked uptake of 2NBDG but 6 strains were not able to take in 2NBDG. Five out of these 6 strains showed NBD-Gly uptake.
DOI: 10.1016/s0003-2697(02)00653-x
发表时间: 2003-03-01
影响因子: 2.9
作者:
Amano, T;Hirasawa, K;Shioi, Y
通讯作者: Shioi, Y