Biogenic triamine and tetraamine activate core catalytic ability of Tetrahymena group I ribozyme in the absence of its large activator module
Biogenic triamine and tetraamine activate core catalytic ability of Tetrahymena group I ribozyme in the absence of its large activator module
复制标题
生物源三胺和四胺在缺乏大激活剂模块的情况下激活四膜虫 I 族核酶的核心催化能力
DOI：
10.1016/j.bbrc.2018.01.085
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发表时间：
2018
影响因子：
3.1
通讯作者：
Ikawa Yoshiya
中科院分区：
文献类型：
--
作者：
Gulshan Mst Ara;Rahman Md Motiar;Matsumura Shigeyoshi;Higuchi Tsunehiko;Umezawa Naoki;Ikawa Yoshiya
组I内含子核酶共享共同的核心元件，其形成负责其催化活性的三维结构。这种核心结构是不稳定的，没有其他因素的帮助，稳定其三级结构。我们研究了生物三胺和四胺及其片段对结构不稳定的I组核酶ΔP5的稳定能力，ΔP5核酶是从四膜虫I组内含子核酶中删除其大的激活子模块得到的。生物三胺（亚精胺）和四胺（精胺）有效地激活了ΔP5核酶的条件下，核酶几乎是无活性的。这些观察结果表明，多胺是有前途的小分子调节剂，激活和可能抑制核心催化能力的第一组核酶。
Group I intron ribozymes share common core elements that form a three-dimensional structure responsible for their catalytic activity. This core structure is unstable without assistance from additional factors that stabilize its tertiary structure. We examined biogenic triamine and tetraamine and also their fragments for their abilities to stabilize a structurally unstable group I ribozyme, ΔP5 ribozyme, derived from theTetrahymenagroup I intron ribozyme by deleting its large activator module. Biogenic triamine (spermidine) and tetraamine (spermine) efficiently activated the ΔP5 ribozyme under conditions where the ribozyme was virtually inactive. These observations suggested that polyamines are promising small molecule modulators to activate and possibly inhibit the core catalytic ability of group I ribozymes.