Strategies for automated analysis of C-elegans locomotion
Strategies for automated analysis of C-elegans locomotion
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DOI：
10.1007/s10158-008-0077-3
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发表时间：
2008-09-01
影响因子：
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通讯作者：
Sattelle, David B.
中科院分区：
文献类型：
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作者：
Buckingham, Steven D.;Sattelle, David B.
C.的自动分析。线虫行为是一个迅速发展的领域，提供了基于行为的高通量药物筛选和系统表型分析的可能性。用于蜗杆形状和运动参数化的标准方法正在出现，并且在克服蠕虫之间的相互作用以及蜗杆盘绕和欧米茄车削所带来的困难方面取得了进展。目前的方法已经促进了微妙的表型的识别和表征的神经元在向前运动和趋化性的作用，以及行为选择和昼夜节律的活动模式的定量表征。考虑到C.虽然线虫已经被用于遗传筛选和化学筛选，但人们希望蠕虫追踪器最终可以在测定行为表型方面提供类似的速度。然而，在实现这一目标之前，必须取得相当大的进展。例如，在全基因组RNAi筛选的情况下，蠕虫基因组中存在约19，000个基因，这意味着即使在自动表型分析系统中最小的用户干预也将非常昂贵。尽管如此，最近的进展表明，药物对大量蠕虫的作用是可以追踪的，这增加了高通量行为筛查可能很快可用的希望。
Automated analysis of C. elegans behaviour is a rapidly developing field, offering the possibility of behaviour-based, high-throughput drug screens and systematic phenotyping. Standard methods for parameterizing worm shapes and movements are emerging, and progress has been made towards overcoming the difficulties introduced by interactions between worms, as well as worm coiling and omega turning. Current methods have facilitated the identification of subtle phenotypes and the characterisation of roles of neurones in forward locomotion and chemotaxis, as well as the quantitative characterisation of behaviour choice and circadian patterns of activity. Given the speed with which C. elegans has been deployed in genetic screens and chemical screens, it is to be hoped that wormtrackers may eventually provide similar rapidity in assaying behavioural phenotypes. However, considerable progress must be made before this can be accomplished. In the case of genome-wide RNAi screens, for example, the presence in the worm genome of some 19,000 genes means that even the minimal user intervention in an automatic phenotyping system will be very costly. Nonetheless, recent advances have shown that drug actions on large numbers of worms can be tracked, raising hopes that high-throughput behavioural screens may soon be available.