Methacrylate-ended polypeptides and polypeptoids for antimicrobial and antifouling coatings
Methacrylate-ended polypeptides and polypeptoids for antimicrobial and antifouling coatings
复制标题
用于抗菌和防污涂层的甲基丙烯酸酯末端多肽和类多肽
DOI：
10.1039/c7py01495c
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发表时间：
2017-11-07
影响因子：
4.6
通讯作者：
Ma, Peter X.
中科院分区：
文献类型：
--
作者：
Gao, Qiang;Li, Peng;Ma, Peter X.
通过N-羧基氰基丙烯酸酯（NCA）的开环聚合（ROP）成功地合成了甲基丙烯酸酯封端的多肽/类多肽。这些低聚物在紫外线（UV）照射下通过聚多巴胺（pDA）层进一步引发，所述聚多巴胺（pDA）层可附接到几乎所有材料的表面以产生聚合物刷涂层。这种包含阳离子抗微生物多肽（MePpep）和多聚肌氨酸（MePsar）的刷状聚合物涂层对四种病原体（金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌和白色念珠菌）表现出有效的抗微生物活性，以及在抵抗蛋白质和血小板粘附方面的抗微生物活性，并因此防止生物膜形成长达7天。体外细胞毒性研究表明，该涂层与小鼠成纤维细胞（L929）具有生物相容性。更重要的是，该涂层在体内表现出显著的抗感染性。这种双功能聚合物刷涂层可以通过贻贝启发的pDA涂层固定在多种材料的表面上，因此应该广泛适用于许多类别的生物医学材料中的抗感染。
Methacrylate-ended polypeptides/polypeptoids were successfully synthesized via ring-opening polymerization (ROP) of N-carboxyanhydrides (NCA). These oligomers were further initiated under ultraviolet (UV) irradiation by a polydopamine (pDA) layer which is attachable to the surface of virtually all materials to generate a polymer brush coating. This brush-like polymer coating comprising cationic antimicrobial polypeptides (MePpep) and antifouling polysarcosine (MePsar) exhibited effective antimicrobial activity against four pathogens (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans), as well as antifouling activity in the resistance to protein and platelet adhesion, and thus prevented biofilm formation for up to 7 days. An in vitro cytotoxicity study showed that this coating is biocompatible with mouse fibroblast (L929) cells. More importantly, this coating exhibited significant anti-infectivity in vivo. This dual-functional polymer brush coating can be immobilized on the surface of multiple categories of materials through the mussel-inspired pDA coating, and thus should be widely applicable for combating infection in many classes of bio-medical materials.