Gene expression of appetite-related hormones: Responses to fasting in different brain regions of yellowtail Seriola quinqueradiata
Gene expression of appetite-related hormones: Responses to fasting in different brain regions of yellowtail Seriola quinqueradiata
复制标题
DOI：
10.1007/s12562-022-01654-6
复制
发表时间：
2022-12
影响因子：
1.9
通讯作者：
Ayaka Senzui;H. Fukada
中科院分区：
文献类型：
--
作者：
Ayaka Senzui;H. Fukada
鱼的食欲由食欲激素以复杂的方式调节。为了研究食欲调节，在日本最重要的水产养殖品种鰤鱼的八个大脑区域中观察了食欲相关激素基因的禁食反应。在嗅球、端脑、视神经中测量食欲相关激素、神经肽 Y (NPY)、两种刺鼠相关蛋白（AgRP1 和 AgRP2）、两种黑色素浓缩激素（MCH1 和 MCH2）以及五种可卡因和安非他明调节转录物（CART1b、CART2a、CART2b、CART3a 和 CART3b）的基因表达。顶盖、下丘脑、垂体、小脑体、小脑嵴和延髓，并比较禁食 7 天的鱼和喂食 3 小时的鱼。除ofcart2b外，所有与食欲相关的激素的基因表达均在所有大脑区域中检测到。视 tectumagrp1、mch1、mch2、cart1b 的表达；下丘脑agrp1;垂体cart1bandcart3a；禁食后小脑体py、agrp1、agrp2显着升高。禁食显着降低嗅球cart1bandcart2b、视顶盖cart2b、下丘脑cart1b、垂体cart2a和cart3b、小脑体cart2a和延髓cart2和mch2的表达。这些结果表明，除了下丘脑（食欲中心）之外的大脑区域也可能参与食欲调节。
Fish appetite is modulated in a complex manner by orexigenic hormones. To investigate appetite regulation, the fasting response of appetite-related hormone genes in eight brain regions was observed in yellowtailSeriola quinqueradiata, the most important aquaculture species in Japan. Gene expression of appetite-related hormones, neuropeptide Y (NPY), two agouti-related proteins (AgRP1 and AgRP2), two melanin-concentrating hormones (MCH1 and MCH2), and five cocaine- and amphetamine-regulated transcripts (CART1b, CART2a, CART2b, CART3a, and CART3b) was measured in the olfactory bulb, telencephalon, optic tectum, hypothalamus, pituitary gland, corpus cerebellum, crista cerebellum, and medulla oblongata and compared between 7-day-fasted fish and 3-h post-fed fish. Except that ofcart2b, the gene expression of all appetite-related hormones was detected in all brain regions. The expression of optic tectumagrp1,mch1,mch2, andcart1b; hypothalamicagrp1; pituitarycart1bandcart3a; and corpus cerebellarnpy,agrp1, andagrp2was significantly increased after fasting. Fasting significantly reduced olfactory bulbcart1bandcart2b, optic tectumcart2b, hypothalamiccart1b, pituitarycart2aandcart3b, corpus cerebellarcart2a, and medulla oblongataagrp2andmch2expressions. These results suggest that brain regions other than the hypothalamus—the center of appetite—might also be involved in appetite regulation.