Detection by PCR of the VNTR polymorphism at D4S95.
Detection by PCR of the VNTR polymorphism at D4S95.
复制标题
通过 PCR 检测 D4S95 处的 VNTR 多态性。
DOI:
10.1093/nar/19.14.4015-a
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发表时间:
1991
影响因子:
14.9
通讯作者:
Gusella,JF
中科院分区:
文献类型:
--
作者:
Allitto,BA;Horn,GT;Altherr,MR;Richards,B;McClatchey,AI;Wasmuth,JJ;Gusella,JF
Description: STR 14C19 is the double stranded DNA probe of sequence (GGGTGCTCGGGTAC) n obtained by annealing of the oligonucleotide 5'GGGTGCTCGGGTAC3'with the overlapping complementary 5'AGCACCCGTACCCG3'followed by ligation and size selection of fragments above 400 bp, as described previously (1, 2). Polymorphism: HAEIII identifies seven alleles ranging from 4 kb to 10 kb.