Detection by PCR of the VNTR polymorphism at D4S95.

Detection by PCR of the VNTR polymorphism at D4S95.
复制标题

通过 PCR 检测 D4S95 处的 VNTR 多态性。

DOI:
10.1093/nar/19.14.4015-a
复制
发表时间:
1991
影响因子:
14.9
通讯作者:
Gusella,JF
Gusella,JF
中科院分区:
生物学2区
文献类型:
--
作者:
Allitto,BA;Horn,GT;Altherr,MR;Richards,B;McClatchey,AI;Wasmuth,JJ;Gusella,JF

文献摘要

被引文献

相似文献

描述:STR 14C19是序列(GGGTGCTCGGGTAC)n的双链DNA探针,通过将寡核苷酸5‘GGGTGCTCGGGTAC3’与重叠的互补5‘AGCACCCGTACCCG3’退火,然后连接和选择400bp以上的片段,如上所述(1,2)。多态:HAEIII鉴定出7个等位基因,大小从4kb到10kb不等。
Description: STR 14C19 is the double stranded DNA probe of sequence (GGGTGCTCGGGTAC) n obtained by annealing of the oligonucleotide 5'GGGTGCTCGGGTAC3'with the overlapping complementary 5'AGCACCCGTACCCG3'followed by ligation and size selection of fragments above 400 bp, as described previously (1, 2). Polymorphism: HAEIII identifies seven alleles ranging from 4 kb to 10 kb.