REQUIREMENTS FOR IMMUNOGLOBULIN AND THE CLASSICAL AND ALTERNATIVE COMPLEMENT PATHWAYS FOR PHAGOCYTOSIS AND INTRACELLULAR KILLING OF MULTIPLE STRAINS OF GRAM-NEGATIVE AEROBIC BACILLI
REQUIREMENTS FOR IMMUNOGLOBULIN AND THE CLASSICAL AND ALTERNATIVE COMPLEMENT PATHWAYS FOR PHAGOCYTOSIS AND INTRACELLULAR KILLING OF MULTIPLE STRAINS OF GRAM-NEGATIVE AEROBIC BACILLI
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DOI：
10.1128/iai.26.1.99-109.1979
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发表时间：
1979-01-01
影响因子：
3.1
通讯作者：
BJORNSON, AB
中科院分区：
文献类型：
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作者：
LEISTWELSH, P;BJORNSON, AB
确定了正常人多形核白细胞对大肠杆菌、奇异变形杆菌、肺炎克雷伯菌和粘质沙雷氏菌临床分离株的吞噬和细胞内杀伤作用对免疫球蛋白 [Ig] 以及替代和经典补体 [C] 途径的需求。比较了缺乏 Ig 或经典途径活性或两者的人血清促进多形核白细胞吞噬和杀死 13 种细菌菌株的能力。在 13 种微生物中，有 7 种需要 Ig 进行吞噬和杀灭，并且仅利用经典 C 途径。三种需要 Ig 并利用经典途径和替代途径。其他 3 种微生物需要最少的 Ig，并利用替代途径或经典途径或两者兼而有之。没有微生物在 Ig 缺乏的血清中利用替代途径，或者在 Ig 和经典途径活性缺乏的血清中被迫利用该途径。不同属的革兰氏阴性需氧杆菌以及同一物种的菌株之间对 Ig 的需求以及多形核白细胞吞噬和细胞内杀伤的替代和经典 C 途径的需求存在异质性。依赖于最小 Ig 的经典途径激活机制可能参与某些革兰氏阴性需氧杆菌的吞噬作用和细胞内杀伤作用。
Requirements for immunoglobulin [Ig] and the alternative and classical complement [C] pathways for phagocytosis and intracellular killing of clinical isolates of Escherichia coli, Proteus mirabilis, Klebsiella pneumoniae and Serratia marcescens by normal human polymorphonuclear leukocytes were determined. Human sera deficient in Ig or classical pathway activity or both were compared for ability to promote phagocytosis and killing of 13 bacterial strains by the polymorphonuclear leukocytes. Of the 13 microorganisms, 7 required Ig for phagocytosis and killing and utilized only the classical C pathway. Three required Ig and utilized the classical and alternative pathways. The other 3 microorganisms required minimal Ig and utilized the alternative or classical pathway or both. No microorganism utilized the alternative pathway in Ig-deficient sera or could be forced to utilize this pathway in sera deficient in Ig and classical pathway activity. A heterogeneity in the requirements for Ig and the alternative and classical C pathways for phagocytosis and intracellular killing by polymorphonuclear leukocytes was demonstrated among various genera of gram-negative aerobic bacilli and among strains of the same species. A mechanism of classical pathway activation dependent upon minimal Ig may participate in phagocytosis and intracellular killing of certain gram-negative aerobic bacilli.