Prolactin controls branchial clcn2c but not atp1a1a.2 in zebrafish Danio rerio
Prolactin controls branchial clcn2c but not atp1a1a.2 in zebrafish Danio rerio
复制标题
催乳素控制斑马鱼斑马鱼鳃 clcn2c 但不控制 atp1a1a.2
DOI：
10.1111/jfb.13854
复制
发表时间：
2018
影响因子：
2
通讯作者：
Breves, Jason P.
中科院分区：
文献类型：
--
作者：
Breves, Jason P.
本研究检查了窄盐斑马鱼的鳃上皮，特别是表达Na+ -Cl -共转运体- 2 （Slc12a10.2）的离子细胞（Na+ -Cl -共转运体[Ncc] -细胞），其介导淡水环境中离子的主动摄取。该研究评估了垂体激素催乳素（Prl）是否刺激Ncc -细胞中编码Clc Cl -通道家族成员（clcn2c）和Na+ -K + - atp酶α1亚基（atp1a1a.2）的信使(m) rna的表达。branchalclcn2c，但不是p1a1a。在体外和体内均对Prl敏感。这些观察结果表明，Prl通过调节clcn2c有助于维持全身Cl−平衡。
This study examined the branchial epithelium of stenohaline zebrafishDanio rerio, and in particular Na+–Cl−cotransporter‐like 2 (Slc12a10.2)‐expressing ionocytes (Na+–Cl−cotransporter [Ncc]‐cells), which mediate the active uptake of ions from freshwater environments. The study assessed whether the pituitary hormone prolactin (Prl) stimulates the expression of messenger (m)RNAs encoding a Clc Cl−channel family member (clcn2c) and a Na+–K+‐ATPase α1 subunit (atp1a1a.2) expressed in Ncc‐cells. Branchialclcn2c, but notatp1a1a.2levels, were sensitive to Prl bothin vitroandin vivo. These observations suggest that Prl contributes to maintaining systemic Cl−balance via the regulation ofclcn2c.