Substrate adaptation of Trichophyton rubrum secreted endoproteases
Substrate adaptation of Trichophyton rubrum secreted endoproteases
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红色毛癣菌分泌的内切蛋白酶的底物适应
DOI：
10.1016/j.micpath.2009.12.001
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发表时间：
2010-02-01
影响因子：
3.8
通讯作者：
Lai, Wei
中科院分区：
文献类型：
--
作者：
Chen, Jian;Yi, Jinling;Lai, Wei
红色毛霉菌是引起人类指甲和皮肤皮肤真菌病的最常见病原菌。分泌的蛋白酶被认为是最重要的毒力因素。然而，红色毛滴虫分泌性蛋白水解酶的底物适应性在很大程度上是未知的。我们首次以人指甲和皮肤角质层的角蛋白为生长介质，研究了红色毛滴虫分泌内切酶基因的不同表达模式。在两个含角蛋白的培养基中，SUB7和MEP2基因在每个家系中的表达水平最高。这些结果表明，在宿主感染过程中，红色毛滴虫分泌的主要内切酶可能是SUB7和MEP2，其他酶可能起辅助作用。直接比较皮肤和指甲上生长的红色毛滴虫对分泌内切酶的底物有不同的喜好。MEP2、SUB5、SUB2和SUB3基因在皮肤生长过程中活性较高，可能与皮肤原角蛋白有较高的亲和力。而SUB1、SUB4和MEN的结构可能更适合指甲原角蛋白的降解。这项工作为进一步了解分泌性蛋白水解酶的发病机制和毛滴虫的广泛适应性提供了有用的分子细节。(C)2009爱思唯尔有限公司。保留所有权利。
Trichophyton rubrum is the most common pathogen caused the dermatophytosis of nail and skin in human. The secreted proteases were considered to be the most important virulence factors. However, the substrates adaptation of T. rubrum secreted proteases is largely unknown. For the first time, we use the keratins from human nail and skin stratum corneum as the growth medium to investigate the different expression patterns of T. rubrum secreted endoproteases genes. During grow in both keratin-containing media SUB7 and MEP2 were the highest expressed gene in each family. These results indicated that SUB7 and MEP2 may be the dominant endoproteases secreted by T. rubrum during host infection and the other proteases may play a supplementary role. The direct comparison of T. rubrum grown on skin and nail medium showed different substrate favorite of secreted endoproteases. The genes MEP2, SUB5, SUB2 and SUB3 were more active during growth in skin medium, possibly these proteases have a higher affinity for skin original keratins. While the structures of SUB1, SUB4, and MEN maybe more suitable for the degradation of nail original keratins. This work presents useful molecular details for further understanding the pathogenesis of secreted proteases and the wide adaptation of T rubrum. (C) 2009 Elsevier Ltd. All rights reserved.