Measurement of protein sulfenic acid content.
Measurement of protein sulfenic acid content.
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DOI：
10.1002/0471140856.tx1702s38
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发表时间：
2008-11
期刊：
影响因子：
--
通讯作者：
Poole, Leslie B
中科院分区：
文献类型：
--
作者：
Poole, Leslie B
蛋白质磺酸是一种反应性的、可逆氧化的半胱氨酸残基，在氧化还原催化和调节中起作用。蛋白质中这些物种的检测和定量是通过7-氯-4-硝基苯并-2-氧-1，3-二唑(NBD氯)、2-硝基-5-硫代苯甲酸酯(TNB)、双酮或双酮的衍生物等试剂的化学修饰，然后进行紫外-可见光谱或质谱分析来完成的。
Protein sulfenic acids are reactive, reversibly oxidized cysteinyl residues with roles in redox catalysis and regulation. Detection and quantification of these species in proteins is accomplished through chemical modification by reagents such as 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD chloride), 2-nitro-5-thiobenzoate (TNB), dimedone, or derivatives of dimedone, followed by UV-visible spectroscopy or mass spectrometric analysis.