The neural crest- and placodes-derived afferent innervation of the mouse esophagus
The neural crest- and placodes-derived afferent innervation of the mouse esophagus
复制标题
DOI：
10.1111/nmo.12002
复制
发表时间：
2012-10-01
影响因子：
3.5
通讯作者：
Kollarik, M.
中科院分区：
文献类型：
--
作者：
Surdenikova, L.;Ru, F.;Kollarik, M.
小鼠是食道神经机制研究的宝贵模型，但小鼠食道的传入神经支配尚不完全清楚。迷走神经传入神经元来源于两个胚胎来源：神经嵴和鳃外基。我们假设神经嵴和基板都有助于小鼠食道trpv1阳性（潜在的伤害性）迷走神经支配。方法从颈部食管逆行标记迷走颈/结节神经节（JNG）和脊髓背根神经节（DRG）神经元。对标记的神经元进行单细胞RT-PCR。在神经嵴来源细胞中表达报告基因的Wnt1Cre/R26R小鼠中，我们发现神经嵴和placoce来源的迷走神经JNG神经元都支配着小鼠的食道。在野生型小鼠中，食道迷走神经JNG trpv1阳性神经元分为两个亚群：假定的神经冠源性嘌呤能受体p2x2阴性/原肽激肽- a （PPT-A）阳性亚群和假定的placogs源性p2x2阳性/ ppta阴性亚群。这些亚群还通过TrkA和GFRa3在假定的神经嵴衍生亚群中的表达以及TrkB在假定的placogs衍生亚群中的表达来分离。trpv1阳性的食管DRG神经元表型与迷走神经推测的神经嵴衍生亚群相似。支配小鼠食道的trpv1阳性（潜在的伤害性）迷走神经传入神经元来源于神经嵴和基板。神经营养因子受体的表达谱在神经嵴来源的迷走神经和脊髓伤害感受器之间相似，但与迷走神经斑块来源的伤害感受器不同。
Background The mouse is an invaluable model for mechanistic studies of esophageal nerves, but the afferent innervation of the mouse esophagus is incompletely understood. Vagal afferent neurons are derived from two embryonic sources: neural crest and epibranchial placodes. We hypothesized that both neural crest and placodes contribute to the TRPV1-positive (potentially nociceptive) vagal innervation of the mouse esophagus. Methods Vagal jugular/nodose ganglion (JNG) and spinal dorsal root ganglia (DRG) neurons were retrogradely labeled from the cervical esophagus. Single cell RT-PCR was performed on the labeled neurons. Key Results In the Wnt1Cre/R26R mice expressing a reporter in the neural crest-derived cells we found that both the neural crest- and the placodes-derived vagal JNG neurons innervate the mouse esophagus. In the wild-type mouse the esophageal vagal JNG TRPV1-positive neurons segregated into two subsets: putative neural crest-derived purinergic receptor P2X2-negative/preprotachykinin-A (PPT-A)-positive subset and putative placodes-derived P2X2-positive/PPTA-negative subset. These subsets also segregated by the expression of TrkA and GFRa3 in the putative neural crest-derived subset, and TrkB in the putative placodes-derived subset. The TRPV1-positive esophageal DRG neurons had the phenotype similar to the vagal putative neural crest-derived subset. Conclusions & Inferences The TRPV1-positive (potentially nociceptive) vagal afferent neurons innervating the mouse esophagus originate from both neural crest and placodes. The expression profile of the receptors for neurotrophic factors is similar between the neural crest-derived vagal and spinal nociceptors, but distinct from the vagal placodes-derived nociceptors.