RCN1/OsABCG5, an ATP-binding cassette (ABC) transporter, is required for hypodermal suberization of roots in rice (Oryza sativa).-猫眼课题宝

DOI：

10.1111/tpj.12614

发表时间：

2014-10

期刊：

The Plant journal : for cell and molecular biology

影响因子：

通讯作者：

中科院分区：

其他

文献类型：

作者：

K. Shiono;Miho Ando;S. Nishiuchi;H. Takahashi;Kohtaro Watanabe;Motoaki Nakamura;Yuichi Matsuo;Naoko Yasuno;U. Yamanouchi;Masaru Fujimoto;H. Takanashi;Kosala Ranathunge;R. Franke;N. Shitan;N. Nishizawa;I. Takamure;M. Yano;N. Tsutsumi;L. Schreiber;K. Yazaki;M. Nakazono;K. Kato

桉叶素是一种由脂肪族和酚类化合物组成的复杂聚合物。它是植物质外体界面的组成部分。在许多植物物种中，包括水稻（Oryza sativa），根外部的皮下组织形成栓化细胞壁（凯氏带和/或木栓质层），其抑制水和离子的流动并保护免受病原体的侵害。迄今为止，没有遗传学证据表明木栓质在下皮中形成质外体转运屏障。我们发现水稻减少秆数1（rcn 1）突变体在淹水土壤中不能产生超过100 mm的根。该突变基因编码一个ATP结合盒（ABC）转运蛋白，命名为RCN 1/OsABCG 5。RCN 1/OsABCG 5基因在野生型中的表达在停滞缺氧条件下在大多数下皮层和一些内皮层根细胞中增加。GFP-RCN 1/OsABCG 5融合蛋白定位于野生型的质膜上。在停滞缺氧条件下，良好的木栓化下皮发达的野生型，但不是在rcn 1突变体。在停滞缺氧条件下，质外体示踪剂（高碘酸和小檗碱）在野生型的下皮层被阻断，但在rcn 1中没有，表明突变体的质外体屏障受损。来自C（28）和C（30）脂肪酸或ω-OH脂肪酸的主要脂族木栓素单体的量在rcn 1中比野生型低得多。这些结果表明，RCN 1/OsABCG 5在水稻根的下皮层的栓化中发挥作用，这有助于质外体屏障的形成。

Suberin is a complex polymer composed of aliphatic and phenolic compounds. It is a constituent of apoplastic plant interfaces. In many plant species, including rice (Oryza sativa), the hypodermis in the outer part of roots forms a suberized cell wall (the Casparian strip and/or suberin lamellae), which inhibits the flow of water and ions and protects against pathogens. To date, there is no genetic evidence that suberin forms an apoplastic transport barrier in the hypodermis. We discovered that a rice reduced culm number1 (rcn1) mutant could not develop roots longer than 100 mm in waterlogged soil. The mutated gene encoded an ATP-binding cassette (ABC) transporter named RCN1/OsABCG5. RCN1/OsABCG5 gene expression in the wild type was increased in most hypodermal and some endodermal roots cells under stagnant deoxygenated conditions. A GFP-RCN1/OsABCG5 fusion protein localized at the plasma membrane of the wild type. Under stagnant deoxygenated conditions, well suberized hypodermis developed in wild types but not in rcn1 mutants. Under stagnant deoxygenated conditions, apoplastic tracers (periodic acid and berberine) were blocked at the hypodermis in the wild type but not in rcn1, indicating that the apoplastic barrier in the mutant was impaired. The amount of the major aliphatic suberin monomers originating from C(28) and C(30) fatty acids or ω-OH fatty acids was much lower in rcn1 than in the wild type. These findings suggest that RCN1/OsABCG5 has a role in the suberization of the hypodermis of rice roots, which contributes to formation of the apoplastic barrier.

RCN1/OsABCG5, an ATP-binding cassette (ABC) transporter, is required for hypodermal suberization of roots in rice (Oryza sativa).

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