Evaluation of the bactec MGIT 960 TB system for recovery and identification of Mycobacterium tuberculosis complex in a high volume tertiary care centre
Evaluation of the bactec MGIT 960 TB system for recovery and identification of Mycobacterium tuberculosis complex in a high volume tertiary care centre
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DOI：
10.4103/0255-0857.53203
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发表时间：
2009-07-01
影响因子：
1.6
通讯作者：
Mehta, A.
中科院分区：
文献类型：
--
作者：
Rodrigues, C.;Shenai, S.;Mehta, A.
目的：评估自动化 BACTEC MGIT 960（一种用于培养结核分枝杆菌复合体的非放射性、非侵入性液体培养系统）在恢复率和时间方面的性能。材料和方法：从2005年3月到2007年12月，使用MGIT 960系统处理了14,597个标本，并将结果与常规L.J培养基进行了比较。我们对 MGIT 960 TB 系统上的对硝基苯甲酸 (PNBA) 测定进行了标准化，以鉴定结核分枝杆菌复合体，并通过将结果与内部分子测定和测序进行比较来评估其有效性。结果与讨论：在总共 6143 株（42 株）结核分枝杆菌复合体阳性分离株中，MGIT 960 TB 系统检测出 6015 株（41 株）呈阳性。相比之下，3526 (24) 个结核分枝杆菌复合体分离株在传统 L.J 培养基上生长。涂片阳性标本中分枝杆菌生长的平均周转时间对于 MGIT 960 为 9 天，对于 L.J. 培养基为 38 天，而在涂片阴性标本中，MGIT 为 16 天，而 L.J. 为 48 天。 结论：MGIT 960 系统与 PNBA 测定法一起鉴定结核分枝杆菌复合体，是实验室处理大量标本的一种快速且有用的方法。
Aim: To evaluate the performance of an automated BACTEC MGIT 960, a non-radioactive, non-invasive liquid culture system for cultivation of M. tuberculosis complex in terms of recovery rate and time. Materials and Methods: From March 2005 to December 2007, 14,597 specimens were processed using the MGIT 960 system and the results were compared with conventional L.J medium. We standardised r-nitro benzoic acid (PNBA) assay on MGIT 960 TB system for identification of M. tuberculosis complex and evaluated its usefulness by comparing the results with an in-house molecular assay and sequencing. Results and Discussion: Of the total 6143 (42) isolates positive for M. tuberculosis complex, 6015 (41) were positive by MGIT 960 TB system. In contrast, 3526 (24) M. tuberculosis complex isolates grew on the conventional L.J medium. The mean turn around time for mycobacterial growth in smear-positive specimens was nine days for MGIT 960, and 38 days for L.J. medium whereas in smear negative specimens it was 16 days by MGIT vs. 48 days by L.J. Conclusion: MGIT 960 system with PNBA assay for identification of M. tuberculosis complex is a rapid and useful method in laboratories processing a large number of specimens.