Catechin degradation with concurrent formation of homo- and heterocatechin dimers during in Vitro digestion
Catechin degradation with concurrent formation of homo- and heterocatechin dimers during in Vitro digestion
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DOI：
10.1021/jf071645m
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发表时间：
2007-10-31
影响因子：
6.1
通讯作者：
Ferruzzi, Mario G.
中科院分区：
文献类型：
--
作者：
Neilson, Andrew P.;Hopf, Amber S.;Ferruzzi, Mario G.
儿茶素进行体外胃和小肠消化。在所有测试浓度下，EGCG、EGC和ECG均显著降解，损失分别为71- 91%、72- 100%和60- 61%。EC和C相对稳定，分别损失8- 11%和7- 8%。HLPC-ESI-MS/MS分析表明，EGCG在模拟消化条件下降解，产生了自氧化的茶氨酸（THSNs）A和D（m/z 913）以及P-2（m/z 883）。EGC二聚化产生同二聚体THSN C和E（m/z 609）和类似于P-2的同二聚体（m/z 579）。未观察到ECG同源二聚体。EGCG和EGC形成类似于THSN（m/z 761）和P-2（m/z 731）的异二聚体。EGCG和ECG形成类似于THSN的同源二聚体（m/z 897）。这项研究提供了一个扩展的配置文件的儿茶素二聚体的消化来源，可能会形成以下消费的儿茶素。这些数据为初步筛选儿茶素体内消化产物提供了逻辑依据。
Catechins were subjected to in vitro gastric and small intestinal digestion. EGCG, EGC, and ECG were significantly degraded at all concentrations tested, with losses of 71-91, 72-100, and 60-61 %, respectively. EC and C were comparatively stable, with losses of 8-11 and;7-8%, respectively. HLPC-ESI-MS/MS indicated that EGCG degradation under simulated digestion resulted in production of theasinensins (THSNs) A and D (m/z 913) and P-2 (m/z 883), its autoxidation homodimers. EGC dimerization produced the homodimers THSN C and E (mlz 609) and homodimers analogous to P-2 (mlz 579). ECG homodimers were not observed. EGCG and EGC formed heterodimers analogous to the THSNs (mlz 761) and P-2 (mlz 731). EGCG and ECG formed homodimers analogous to the THSNs (m/z 897). This study provides an expanded profile of catechin dimers of digestive origin that may potentially form following consumption of catechins. These data provide a logical basis for initial screening to detect catechin digestive products in vivo.