Structure elucidation of the O-antigen of Salmonella enterica O51 and its structural and genetic relation to the O-antigen of Escherichia coli O23
Structure elucidation of the O-antigen of Salmonella enterica O51 and its structural and genetic relation to the O-antigen of Escherichia coli O23
复制标题
DOI：
10.1134/s0006297911070078
复制
发表时间：
2011-12
期刊：
影响因子：
--
通讯作者：
A. Perepelov;B. Liu;Dan Guo;S. Senchenkova;A. S. Shahskov;Lu Feng;Lei Wang;Y. Knirel
中科院分区：
文献类型：
--
作者：
A. Perepelov;B. Liu;Dan Guo;S. Senchenkova;A. S. Shahskov;Lu Feng;Lei Wang;Y. Knirel
肠炎沙门氏菌O51的O-多糖(O-抗原)是通过对脂多糖的温和酸解分离出来的，其结构经糖分析和核磁共振波谱确证。大肠埃希氏菌的O-抗原具有相似的结构，只是在主链上α-D-Glc残基的第6位增加了一个侧链Glc-D-Glc。O-抗原基因簇OFS的测序。EntricaO51 Ande。ColiO23揭示了相同的基因，具有高度的相似性。通过与开放的基因数据库的比较，所有预期合成两个O-抗原共同结构的基因都被赋予了功能。推测这两种细菌的基因簇起源于同一祖先，而O-抗原修饰基因则不同。ColiO23很可能是由基因簇外的原噬菌体基因诱导的，可能在物种分化后引入。
The O-polysaccharide (O-antigen) ofSalmonella entericaO51 was isolated by mild acid degradation of the lipopolysaccharide and its structure was established using sugar analysis and NMR spectroscopy. The O-antigen ofEscherichia coliO23, whose structure was elucidated earlier, possesses a similar structure and differs only in the presence of an additional lateral α-D-Glcpresidue at position 6 of the GlcNAc residue in the main chain. Sequencing of the O-antigen gene clusters ofS. entericaO51 andE. coliO23 revealed the same genes with a high-level similarity. By comparison with opened gene databases, all genes expected for the synthesis of the common structure of the two O-antigens were assigned functions. It is suggested that the gene clusters of both bacteria originated from a common ancestor, whereas the O-antigen modification inE. coliO23, which, most probably, is induced by prophage genes outside the gene cluster, could be introduced after the species divergence.