1950 MHz IMT-2000 Field Does Not Activate Microglial Cells In Vitro
1950 MHz IMT-2000 Field Does Not Activate Microglial Cells In Vitro
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DOI：
10.1002/bem.20532
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发表时间：
2010-02-01
影响因子：
1.9
通讯作者：
Ugawa, Yoshikazu
中科院分区：
文献类型：
--
作者：
Hirose, Hideki;Sasaki, Atsushi;Ugawa, Yoshikazu
鉴于当今手机的广泛使用，对射频（RF）场的潜在生物效应的研究变得越来越重要。特别是，许多研究已经进行了RF对大脑功能的影响。为了检查由国际移动的电信-2000（IMT-2000）细胞系统控制的1950 MHz调制信号对中枢神经系统（CNS）诱导的任何生物学效应，我们研究了RF场对大脑中小胶质细胞的影响。我们通过检查暴露于1950 MHz宽带码分多址（W-CDMA）RF场（比吸收率（SAR）为0.2）后免疫反应相关分子表达和细胞因子产生的变化来评估小胶质细胞的功能变化。0.8和2.0 W/kg。将从新生大鼠制备的原代小胶质细胞培养物置于RF或假场2小时。以盲法处理暴露后24和72 h获得的试验样品。结果表明，主要组织相容性复合体（MHC）II类，这是激活的小胶质细胞的最常见的标志物，细胞暴露于W-CDMA辐射和假暴露对照之间的细胞阳性的百分比相似。在任何RF场暴露组和假暴露对照组之间，在MHC I类阳性细胞百分比方面均未观察到统计学显著差异。进一步.在暴露于W-CDMA信号的测试组和假暴露的阴性对照之间没有观察到肿瘤坏死因子-α（TNF-α）、白细胞介素-1 β（IL-1 β）和白细胞介素-6（IL-6）的产生的显著差异。这些发现表明，暴露于高达2 W/kg的RF场不会激活体外小胶质细胞。Bioelectromagnetics 31：104-112，2010. (C)2009年威利-利斯。Inc.
Given the widespread use of the cellular phone today, investigation of potential biological effects of radio frequency (RF) fields has become increasingly important. In particular, much research has been conducted on RF effects on brain function. To examine any biological effects on the central nervous system (CNS) induced by 1950 MHz modulation signals, which are controlled by the International Mobile Telecommunication-2000 (IMT-2000) cellular system, we investigated the effect of RF fields on microglial cells in the brain. We assessed functional changes in microglial cells by examining changes in immune reaction-related molecule expression and cytokine production after exposure to a 1950 MHz Wideband Code Division Multiple Access (W-CDMA) RF field, at specific absorption rates (SARs) of 0.2. 0.8, and 2.0 W/kg. Primary microglial cell cultures prepared from neonatal rats were subjected to an RF or sham field for 2 h. Assay samples obtained 24 and 72 h after exposure were processed in a blind manner. Results showed that the percentage of cells positive for major histocompatibility complex (MHC) class II, which is the most common marker for activated microglial cells, was similar between cells exposed to W-CDMA radiation and sham-exposed controls. No statistically significant differences were observed between any of the RF field exposure groups and the sham-exposed controls in percentage of MHC class It positive cells. Further. no remarkable differences in the production of tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), and interleukin-6 (IL-6) were observed between the test groups exposed to W-CDMA signal and the sham-exposed negative controls. These findings suggest that exposure to RF fields up to 2 W/kg does not activate microglial cells in vitro. Bioelectromagnetics 31:104-112, 2010. (C) 2009 Wiley-Liss. Inc.