Biological Evaluation of DNA Damage in Bacteriophages Inactivated by Atmospheric Pressure Cold Plasma
Biological Evaluation of DNA Damage in Bacteriophages Inactivated by Atmospheric Pressure Cold Plasma
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DOI：
10.1002/ppap.200900088
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发表时间：
2010-03
影响因子：
3.5
通讯作者：
H. Yasuda;T. Miura;H. Kurita;K. Takashima;A. Mizuno
中科院分区：
文献类型：
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作者：
H. Yasuda;T. Miura;H. Kurita;K. Takashima;A. Mizuno
等离子体处理微生物的灭活机理仍然是主要的研究课题。我们开发了一种生物测定法，用于评价在空气中用非热大气压等离子体处理的病毒的体内DNA损伤。在中性pH和接近室温的条件下，将不同剂量的等离子体应用于λ噬菌体颗粒的湿态。从处理的λ π ι的每个样品中，纯化DNA并进行体外DNA包装反应。与先前从经处理的猪的存活曲线获得的D值（D= 3 s）相比，重新包装的猪的存活曲线显示出极大的D值（D= 25 s）。结果表明，DNA损伤几乎没有导致失活的证据。
Inactivation mechanisms of plasma treated microorganisms are still major subjects. We have developed a biological assay which evaluates in vivo DNA damage of the viruses treated with non‐thermal atmospheric pressure plasma in air. Different doses of the plasma were applied to wet state of λ phage particles under neutral pH and near the room temperature. From each sample of treated λ phages, DNA was purified and subjected to in vitro DNA packaging reactions. Survival curves of the re‐packaged phages showed an extremely largeDvalue (D= 25 s) compared to the previousDvalue (D= 3 s) obtained from the survival curves of the treated phages. The results indicate the evidence that DNA damage hardly contributed to the inactivation.