Robust hepatitis C virus infection in vitro
Robust hepatitis C virus infection in vitro
复制标题
DOI：
10.1073/pnas.0503596102
复制
发表时间：
2005-06-28
影响因子：
11.1
通讯作者：
Chisari, FV
中科院分区：
文献类型：
--
作者：
Zhong, J;Gastaminza, P;Chisari, FV
缺乏一个强大的丙型肝炎病毒（HCV）感染的细胞培养模型，严重限制了HCV生命周期的分析和有效的抗病毒药物和疫苗的开发。在这里，我们报告了一个简单而强大的HCV细胞培养感染系统的基础上，HCV JFH-1分子克隆和Huh-7衍生的细胞系，允许生产的病毒，可以有效地在组织培养繁殖。该系统为分析宿主-病毒相互作用提供了强有力的工具，这将有助于发现针对这种重要的人类病原体的抗病毒药物和疫苗。
The absence of a robust cell culture model of hepatitis C virus (HCV) infection has severely limited analysis of the HCV life cycle and the development of effective antivirals and vaccines. Here we report the establishment of a simple yet robust HCV cell culture infection system based on the HCV JFH-1 molecular clone and Huh-7-derived cell lines that allows the production of virus that can be efficiently propagated in tissue culture. This system provides a powerful tool for the analysis of host-virus interactions that should facilitate the discovery of antiviral drugs and vaccines for this important human pathogen.