Age-related changes in intracellular cytokine profiles and Th2 dominance in allergic children
Age-related changes in intracellular cytokine profiles and Th2 dominance in allergic children
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DOI：
10.1111/j.1399-3038.2005.00363.x
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发表时间：
2006-03-01
影响因子：
4.4
通讯作者：
Kondo, N
中科院分区：
文献类型：
--
作者：
Kawamoto, N;Kaneko, H;Kondo, N
在过敏性疾病中已经指出了不平衡的T辅助反应。特别是在儿童时期，重要的是要考虑获得性免疫的发展。我们研究了年龄与Th 1、Th 2、Tc 1和Tc 2细胞的关系。此外，将过敏性疾病中的Th 1、Th 2、Tc 1或Tc 2细胞与对照组进行比较。研究对象为34名健康对照（0-40岁）、200份脐血、9名1-3岁特应性皮炎（AD）患者和5名2-6岁支气管哮喘（BA）患者。用CD 4、CD 8进行表面染色，用抗干扰素-γ（IFN-γ）和抗白细胞介素（IL）-4进行细胞内染色，并通过使用流式细胞术进行分析。在健康对照组中，Th 1、Tc 1和Th 2的百分比与年龄呈正相关。Th 1和Tc 1的绝对值也与年龄相关。有过敏性疾病家族史的脐带血与无家族史的脐带血相比无显著差异。AD和BA患者的Th 2细胞百分比显著高于年龄匹配的健康对照组。Th 1、Th 2和Tc 1随年龄的增加可能反映了获得性免疫的发展。在评估T细胞的细胞因子谱时，年龄匹配很重要。在过敏性疾病中，虽然脐带血表现出Th 1-优势模式，但在儿童时期转变为Th 2优势，这可能反映了一些遗传背景。
The unbalanced T helper response has been pointed out in allergic diseases. Especially in childhood, it is important to consider the development of acquired immunity. We investigated the relationship between age and Th1, Th2, Tc1 or Tc2 cells. In addition, Th1, Th2, Tc1 or Tc2 cells in allergic diseases were compared with control subjects. Thirty-four healthy controls (0-40 years old), 200 samples of cord blood, nine patients with atopic dermatitis (AD) (1-3 years old) and five patients with bronchial asthma (BA) (2-6 years old) were studied. Surface staining with CD4, CD8 and intracellular staining with anti-interferon-gamma (IFN-gamma) and anti-interleukin (IL)-4 were carried out, and analyzed by using flow cytometry. In the healthy controls, the percentages of Th1, Tc1 or Th2 showed positive correlation with age. The absolute numbers of Th1 or Tc1 also correlated with age. Cord blood with a family history of allergic disease showed no significant difference compared to that without a family history. The percentage of Th2 in AD and BA patients was significantly higher than in the age-matched healthy controls. The increase in Th1, Th2 and Tc1 with age might reflect on the development of acquired immunity. Age matching is important when evaluating the cytokine profiles of T cells. In allergic diseases, although cord blood showed a Th1-dominant pattern, it changed to Th2 dominance in childhood, and this may reflect on some genetic background.