The tobacco mosaic virus particle: structure and assembly
The tobacco mosaic virus particle: structure and assembly
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DOI：
10.1098/rstb.1999.0404
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发表时间：
1999-03-29
期刊：
影响因子：
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通讯作者：
Klug, A
中科院分区：
文献类型：
--
作者：
Klug, A
简要介绍了物理和化学研究，这些研究导致了对烟草花叶病毒颗粒结构的了解，并且吹它是由其组分外壳蛋白和RNA组装而成的。组装是一个复杂得多的过程，比从粒子的螺旋设计的简单性所预期的要复杂得多。蛋白质形成一个强制性的中间体（由两层蛋白质单元组成的圆柱形圆盘），它识别特定的RNA发夹序列。这种非凡的机制同时满足了使螺旋颗粒生长成核的物理要求和特异性识别病毒DNA的生物学要求。
A short account is given of the physical and chemical studies that have led to an understanding of the structure of the tobacco mosaic virus particle and Blow it is assembled from its constituent coat protein and RNA. The assembly is a much more complex process than might have been expected from the simplicity of the helical design of the particle. The protein forms an obligatory intermediate (a cylindrical disk composed of two layers of protein units), which recognizes a specific RNA hairpin sequence. This extraordinary mechanism simultaneously fulfils the physical requirement for nucleating the growth of the helical particle and the biological requirement for specific recognition of the viral DNA.